Human B cell lines secreting IgM antibody specific for myelin basic protein
In this study we describe for the first time the production of stable human B cell lines and clones that secrete IgM antibody specific for human myelin basic protein. The technique based on limiting dilutions of Epstein-Barr virus-transformed peripheral B cells from patients with multiple sclerosis,...
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| Veröffentlicht in: | Journal of neuroimmunology 1989-09, Vol.24 (1), p.9-16 |
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| container_title | Journal of neuroimmunology |
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| creator | Jingwu, Zhang Lambrechts, Jos Heyligen, Harry Vandenbark, Arthur A. Raus, Jef C.M. |
| description | In this study we describe for the first time the production of stable human B cell lines and clones that secrete IgM antibody specific for human myelin basic protein. The technique based on limiting dilutions of Epstein-Barr virus-transformed peripheral B cells from patients with multiple sclerosis, precluded the need for preselecting or stimulating antigen-specific B cells. Most of the cell lines were stable for at least 6 months in continuous culture and produced 5–12 μg/ml antibody after 2 weeks in culture. The myelin basic protein-specific B cells were surface IgM positive, and occured with a frequency of approximately 1/2500 mononuclear cells in peripheral blood. The successful selection and quantitation of specific B cell clones described here suggests that this technique is well suited for evaluating B cell responses to known and suspected antigens and autoantigens. |
| doi_str_mv | 10.1016/0165-5728(89)90092-1 |
| format | Article |
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The technique based on limiting dilutions of Epstein-Barr virus-transformed peripheral B cells from patients with multiple sclerosis, precluded the need for preselecting or stimulating antigen-specific B cells. Most of the cell lines were stable for at least 6 months in continuous culture and produced 5–12 μg/ml antibody after 2 weeks in culture. The myelin basic protein-specific B cells were surface IgM positive, and occured with a frequency of approximately 1/2500 mononuclear cells in peripheral blood. 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Psychology ; Herpesvirus 4, Human - physiology ; Humans ; Immunoglobulin M - biosynthesis ; Immunoglubin M-secreting B lymphoblastoid cell line ; Kinetics ; Molecular and cellular biology ; Myelin basic protein ; Myelin Basic Protein - immunology</subject><ispartof>Journal of neuroimmunology, 1989-09, Vol.24 (1), p.9-16</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c366t-58168895e0c720b680fd57d6f82f9c4001779d53276790af7717c3ddc96749033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0165-5728(89)90092-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6781449$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2478580$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jingwu, Zhang</creatorcontrib><creatorcontrib>Lambrechts, Jos</creatorcontrib><creatorcontrib>Heyligen, Harry</creatorcontrib><creatorcontrib>Vandenbark, Arthur A.</creatorcontrib><creatorcontrib>Raus, Jef C.M.</creatorcontrib><title>Human B cell lines secreting IgM antibody specific for myelin basic protein</title><title>Journal of neuroimmunology</title><addtitle>J Neuroimmunol</addtitle><description>In this study we describe for the first time the production of stable human B cell lines and clones that secrete IgM antibody specific for human myelin basic protein. The technique based on limiting dilutions of Epstein-Barr virus-transformed peripheral B cells from patients with multiple sclerosis, precluded the need for preselecting or stimulating antigen-specific B cells. Most of the cell lines were stable for at least 6 months in continuous culture and produced 5–12 μg/ml antibody after 2 weeks in culture. The myelin basic protein-specific B cells were surface IgM positive, and occured with a frequency of approximately 1/2500 mononuclear cells in peripheral blood. The successful selection and quantitation of specific B cell clones described here suggests that this technique is well suited for evaluating B cell responses to known and suspected antigens and autoantigens.</description><subject>Antibody Specificity</subject><subject>B-Lymphocytes - immunology</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cell physiology</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>Cell Transformation, Viral</subject><subject>Clone Cells</subject><subject>Epstein-Barr virus transformation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Herpesvirus 4, Human - physiology</subject><subject>Humans</subject><subject>Immunoglobulin M - biosynthesis</subject><subject>Immunoglubin M-secreting B lymphoblastoid cell line</subject><subject>Kinetics</subject><subject>Molecular and cellular biology</subject><subject>Myelin basic protein</subject><subject>Myelin Basic Protein - immunology</subject><issn>0165-5728</issn><issn>1872-8421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtrWzEQRkVpSJ20_6AFLUpIFjeVdPXcBJKQh4lLN-layNIoqNyHK10H_O8jx8bLdjEIZs6MPg5CXym5pITKH7VEIxTT59pcGEIMa-gHNKNasUZzRj-i2QH5hE5K-UMIFS03x-iYcaWFJjP09Lju3YBvsIeuw10aoOACPsOUhhc8f_mJ3TCl5Rg2uKzAp5g8jmPG_QYqjJeu1MYqjxOk4TM6iq4r8GX_nqLf93fPt4_N4tfD_PZ60fhWyqkRmkqtjQDiFSNLqUkMQgUZNYvG8xpSKRNEy5RUhrioFFW-DcEbqbghbXuKznZ3679_11Am26eyze8GGNfFKsMMF0b8F6SCE64lqSDfgT6PpWSIdpVT7_LGUmK3su3WpN2atNrYd9mW1rVv-_vrZQ_hsLS3W-ff93NXvOtidoNP5YBJpSnnpmJXOwyqtNcE2RafYPAQUgY_2TCmf-d4A7mhmFk</recordid><startdate>19890901</startdate><enddate>19890901</enddate><creator>Jingwu, Zhang</creator><creator>Lambrechts, Jos</creator><creator>Heyligen, Harry</creator><creator>Vandenbark, Arthur A.</creator><creator>Raus, Jef C.M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TK</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19890901</creationdate><title>Human B cell lines secreting IgM antibody specific for myelin basic protein</title><author>Jingwu, Zhang ; Lambrechts, Jos ; Heyligen, Harry ; Vandenbark, Arthur A. ; Raus, Jef C.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-58168895e0c720b680fd57d6f82f9c4001779d53276790af7717c3ddc96749033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Antibody Specificity</topic><topic>B-Lymphocytes - immunology</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cell physiology</topic><topic>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</topic><topic>Cell Transformation, Viral</topic><topic>Clone Cells</topic><topic>Epstein-Barr virus transformation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Herpesvirus 4, Human - physiology</topic><topic>Humans</topic><topic>Immunoglobulin M - biosynthesis</topic><topic>Immunoglubin M-secreting B lymphoblastoid cell line</topic><topic>Kinetics</topic><topic>Molecular and cellular biology</topic><topic>Myelin basic protein</topic><topic>Myelin Basic Protein - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jingwu, Zhang</creatorcontrib><creatorcontrib>Lambrechts, Jos</creatorcontrib><creatorcontrib>Heyligen, Harry</creatorcontrib><creatorcontrib>Vandenbark, Arthur A.</creatorcontrib><creatorcontrib>Raus, Jef C.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroimmunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jingwu, Zhang</au><au>Lambrechts, Jos</au><au>Heyligen, Harry</au><au>Vandenbark, Arthur A.</au><au>Raus, Jef C.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human B cell lines secreting IgM antibody specific for myelin basic protein</atitle><jtitle>Journal of neuroimmunology</jtitle><addtitle>J Neuroimmunol</addtitle><date>1989-09-01</date><risdate>1989</risdate><volume>24</volume><issue>1</issue><spage>9</spage><epage>16</epage><pages>9-16</pages><issn>0165-5728</issn><eissn>1872-8421</eissn><coden>JNRIDW</coden><abstract>In this study we describe for the first time the production of stable human B cell lines and clones that secrete IgM antibody specific for human myelin basic protein. The technique based on limiting dilutions of Epstein-Barr virus-transformed peripheral B cells from patients with multiple sclerosis, precluded the need for preselecting or stimulating antigen-specific B cells. Most of the cell lines were stable for at least 6 months in continuous culture and produced 5–12 μg/ml antibody after 2 weeks in culture. The myelin basic protein-specific B cells were surface IgM positive, and occured with a frequency of approximately 1/2500 mononuclear cells in peripheral blood. The successful selection and quantitation of specific B cell clones described here suggests that this technique is well suited for evaluating B cell responses to known and suspected antigens and autoantigens.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2478580</pmid><doi>10.1016/0165-5728(89)90092-1</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-5728 |
| ispartof | Journal of neuroimmunology, 1989-09, Vol.24 (1), p.9-16 |
| issn | 0165-5728 1872-8421 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Antibody Specificity B-Lymphocytes - immunology Biological and medical sciences Cell Line Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Cell Transformation, Viral Clone Cells Epstein-Barr virus transformation Fundamental and applied biological sciences. Psychology Herpesvirus 4, Human - physiology Humans Immunoglobulin M - biosynthesis Immunoglubin M-secreting B lymphoblastoid cell line Kinetics Molecular and cellular biology Myelin basic protein Myelin Basic Protein - immunology |
| title | Human B cell lines secreting IgM antibody specific for myelin basic protein |
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