Human B cell lines secreting IgM antibody specific for myelin basic protein
In this study we describe for the first time the production of stable human B cell lines and clones that secrete IgM antibody specific for human myelin basic protein. The technique based on limiting dilutions of Epstein-Barr virus-transformed peripheral B cells from patients with multiple sclerosis,...
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Veröffentlicht in: | Journal of neuroimmunology 1989-09, Vol.24 (1), p.9-16 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | In this study we describe for the first time the production of stable human B cell lines and clones that secrete IgM antibody specific for human myelin basic protein. The technique based on limiting dilutions of Epstein-Barr virus-transformed peripheral B cells from patients with multiple sclerosis, precluded the need for preselecting or stimulating antigen-specific B cells. Most of the cell lines were stable for at least 6 months in continuous culture and produced 5–12 μg/ml antibody after 2 weeks in culture. The myelin basic protein-specific B cells were surface IgM positive, and occured with a frequency of approximately 1/2500 mononuclear cells in peripheral blood. The successful selection and quantitation of specific B cell clones described here suggests that this technique is well suited for evaluating B cell responses to known and suspected antigens and autoantigens. |
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ISSN: | 0165-5728 1872-8421 |
DOI: | 10.1016/0165-5728(89)90092-1 |