Bacteriophage T4 early promoter regions: Consensus sequences of promoters and ribosome-binding sites

Twenty-nine early promoters from bacteriophage T4 and 14 early promoters from bacteriophage T6 were isolated using vector M13HDL17, a promoterless derivative of M13mp8 carrying a linker sequence, the bacteriophage λ-terminator t R1, and the lacZ′ gene including part of its ribosome-binding site. The...

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Veröffentlicht in:	Journal of molecular biology 1989-08, Vol.208 (4), p.517-536
Hauptverfasser:	Liebig, Hans-Dieter, Rüger, Wolfgang
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Binding Sites Biological and medical sciences Cloning, Molecular DNA, Viral - genetics Fundamental and applied biological sciences. Psychology Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutation Promoter Regions, Genetic Ribosomes T-Phages - genetics Terminator Regions, Genetic Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing
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container_title	Journal of molecular biology
container_volume	208
creator	Liebig, Hans-Dieter Rüger, Wolfgang
description	Twenty-nine early promoters from bacteriophage T4 and 14 early promoters from bacteriophage T6 were isolated using vector M13HDL17, a promoterless derivative of M13mp8 carrying a linker sequence, the bacteriophage λ-terminator t R1, and the lacZ′ gene including part of its ribosome-binding site. The consensus sequence for the T4 promoters is: ▪ Ribosome-binding sites of T4 share the sequence: 5′… g. GGAga.. aA.ATGAa . a…3′ The consensus sequence of the T4 early promoter regions is significantly different in sequence and length from that of Escherichia coli promoters. Only one of the promoters detected with vector M13HDL17 resembled a typical bacterial promoter. The high information content raises the possibility that additional proteins recognize and contact nucleotides within the promoter region. All T4 early promoters also carry DNA sequences that could support DNA curving, a structural feature that might contribute to promoter recognition.
doi_str_mv	10.1016/0022-2836(89)90145-9
format	Article
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The consensus sequence for the T4 promoters is: ▪ Ribosome-binding sites of T4 share the sequence: 5′… g. GGAga.. aA.ATGAa . a…3′ The consensus sequence of the T4 early promoter regions is significantly different in sequence and length from that of Escherichia coli promoters. Only one of the promoters detected with vector M13HDL17 resembled a typical bacterial promoter. The high information content raises the possibility that additional proteins recognize and contact nucleotides within the promoter region. 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The consensus sequence for the T4 promoters is: ▪ Ribosome-binding sites of T4 share the sequence: 5′… g. GGAga.. aA.ATGAa . a…3′ The consensus sequence of the T4 early promoter regions is significantly different in sequence and length from that of Escherichia coli promoters. Only one of the promoters detected with vector M13HDL17 resembled a typical bacterial promoter. The high information content raises the possibility that additional proteins recognize and contact nucleotides within the promoter region. All T4 early promoters also carry DNA sequences that could support DNA curving, a structural feature that might contribute to promoter recognition.</description><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>DNA, Viral - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Promoter Regions, Genetic</subject><subject>Ribosomes</subject><subject>T-Phages - genetics</subject><subject>Terminator Regions, Genetic</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. 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Psychology</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Promoter Regions, Genetic</topic><topic>Ribosomes</topic><topic>T-Phages - genetics</topic><topic>Terminator Regions, Genetic</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liebig, Hans-Dieter</creatorcontrib><creatorcontrib>Rüger, Wolfgang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liebig, Hans-Dieter</au><au>Rüger, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacteriophage T4 early promoter regions: Consensus sequences of promoters and ribosome-binding sites</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1989-08-20</date><risdate>1989</risdate><volume>208</volume><issue>4</issue><spage>517</spage><epage>536</epage><pages>517-536</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><coden>JMOBAK</coden><abstract>Twenty-nine early promoters from bacteriophage T4 and 14 early promoters from bacteriophage T6 were isolated using vector M13HDL17, a promoterless derivative of M13mp8 carrying a linker sequence, the bacteriophage λ-terminator t R1, and the lacZ′ gene including part of its ribosome-binding site. The consensus sequence for the T4 promoters is: ▪ Ribosome-binding sites of T4 share the sequence: 5′… g. GGAga.. aA.ATGAa . a…3′ The consensus sequence of the T4 early promoter regions is significantly different in sequence and length from that of Escherichia coli promoters. Only one of the promoters detected with vector M13HDL17 resembled a typical bacterial promoter. The high information content raises the possibility that additional proteins recognize and contact nucleotides within the promoter region. All T4 early promoters also carry DNA sequences that could support DNA curving, a structural feature that might contribute to promoter recognition.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>2810355</pmid><doi>10.1016/0022-2836(89)90145-9</doi><tpages>20</tpages></addata></record>
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subjects	Base Sequence Binding Sites Biological and medical sciences Cloning, Molecular DNA, Viral - genetics Fundamental and applied biological sciences. Psychology Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutation Promoter Regions, Genetic Ribosomes T-Phages - genetics Terminator Regions, Genetic Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing
title	Bacteriophage T4 early promoter regions: Consensus sequences of promoters and ribosome-binding sites
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