Marek's disease virus EcoRI-Q gene (meq) and a small RNA antisense to ICP4 are abundantly expressed in CD4+ cells and cells carrying a novel lymphoid marker, AV37, in Marek's disease lymphomas
N Ross, G O'Sullivan, C Rothwell, G Smith, SC Burgess, M Rennie, LF Lee and TF Davison Institute for Animal Health, Compton Laboratory, Berks, UK. norman.ross@bbsrc.ac.uk Mature lymphomas produced in Rhode Island Red (RIR) chickens infected with the RB1B strain of Marek's disease virus (MD...
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Veröffentlicht in: | Journal of general virology 1997-09, Vol.78 (9), p.2191-2198 |
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Zusammenfassung: | N Ross, G O'Sullivan, C Rothwell, G Smith, SC Burgess, M Rennie, LF Lee and TF Davison
Institute for Animal Health, Compton Laboratory, Berks, UK. norman.ross@bbsrc.ac.uk
Mature lymphomas produced in Rhode Island Red (RIR) chickens infected with
the RB1B strain of Marek's disease virus (MDV) were examined for the
presence of viral DNA and RNA and expression of viral antigens. In situ
hybridization showed that all tumours examined contained viral DNA in areas
of lymphoid infiltration. In 3/5 tumours, there was a correlation between
the number and distribution of cells expressing the Marek's disease EcoRI-Q
gene (meq) and those that carried the lymphoid cell marker AV37. Expression
of the MDV-specific phosphoprotein pp38 was infrequent in lymphomas but
abundant in a splenic tumour which also expressed the viral glycoprotein
gB. Northern blot analysis of lymphocyte fractions purified by
immunoaffinity showed that CD4+ and AV37+ fractions from lymphomas
expressed meq and the small RNA antisense to ICP4 (SAR). The results are
consistent with the notion that transformed cells are CD4+ cells, carrying
the AV37 marker and expressing meq and SAR. |
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ISSN: | 0022-1317 1465-2099 |
DOI: | 10.1099/0022-1317-78-9-2191 |