The ski oncogene induces muscle differentiation in quail embryo cells

Quail embryo cells (QECs) are primary cultures of fibroblastoid cells that become myogenic after infection with avian retroviruses expressing the ski oncogene (SKVs). ski also stimulates proliferation of QECs and induces morphological transformation and anchorage-independent growth. Paradoxically, ski-transformed clones picked from soft agar are capable of muscle differentiation. ski-induced differentiation is essentially indistinguishable from that of uninfected myoblasts in culture with regard to muscle-specific gene expression, commitment, and inhibition by growth factors or other oncogenes. However, ski-induced myoblasts have less stringent requirements for growth and differentiation. Uninfected QECs cannot differentiate and do not express an early marker for the myogenic lineage. Clonal analysis indicates that at least 40% of QECs are converted by ski to differentiating myoblasts. The data suggest that ski induces either the capacity for differentiation in an “incompetent” muscle precursor or the determination of nonmyogenic cells to the myogenic lineage.