Cell adhesion activity of non-specific cross-reacting antigen (NCA) and carcinoembryonic antigen (CEA) expressed on CHO cell surface: Homophilic and heterophilic adhesion

Cell adhesion activity of non-specific cross-reacting antigen (NCA) and carcinoembryonic antigen (CEA) expressed on CHO cell surface: Homophilic and heterophilic adhesion

Cell adhesion activity of carcinoembryonic antigen (CEA) and non-specific cross-reacting antigen (NCA) has been analysed by using CHO cells which had been transfected with cDNAs and are ectopically expressing each antigen on their surface. CEA expressing CHO tended to aggregate easily within 30 min...

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Veröffentlicht in:Biochemical and biophysical research communications 1989-10, Vol.164 (1), p.39-45
Hauptverfasser: Oikawa, Shinzo, Inuzuka, Chikako, Kuroki, Motomu, Matsuoka, Yuji, Kosaki, Goro, Nakazato, Hiroshi
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Antigens - genetics
Antigens - immunology
Antigens, Neoplasm
Biological and medical sciences
Carcinoembryonic Antigen - genetics
Carcinoembryonic Antigen - immunology
Cell Adhesion
Cell Adhesion Molecules - genetics
Cell interactions, adhesion
Cell Line
Cricetinae
Cricetulus
DNA - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression
Glycoproteins - genetics
Glycoproteins - immunology
Molecular and cellular biology
Plasmids
Transfection
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Zusammenfassung:Cell adhesion activity of carcinoembryonic antigen (CEA) and non-specific cross-reacting antigen (NCA) has been analysed by using CHO cells which had been transfected with cDNAs and are ectopically expressing each antigen on their surface. CEA expressing CHO tended to aggregate easily within 30 min after being suspended by trypsinization. Cell adhesion assay between 51Cr labelled cells and monolayered cells showed both homophilic and heterophilic interaction, the extent of which was CEA-CEA⪢CEA-NCA>NCA-NCA. These reactions were completely inhibited by Fab' fragment of anti-CEA antibody. The results strongly suggested that CEA and NCA function as Ca ++ independent cell adhesion molecules by homophilic and heterophilic interactions.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(89)91679-3