Differential regulations of matrix metalloproteinases and tissue inhibitors of metalloproteinases in dermal fibroblasts by dibutyril cyclic AMP

The influence of dibutyril cyclic AMP (dbcAMP) on the gene expression of matrix metalloproteinases (MMP)-1, MMP-2, MMP-3, tissue inhibitors of metalloproteinases (TIMP)-1 and TIMP-2 was investigated in human skin fibroblasts by northern analyses. The treatment of dbcAMP increased MMP-1 and MMP-3 mRN...

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Veröffentlicht in:	Journal of dermatological science 1997-08, Vol.15 (2), p.95-100
Hauptverfasser:	Kuroda, Kei, Shinkai, Hiroshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Bucladesine - pharmacology Cells, Cultured Collagen - genetics Collagenases - genetics dbcAMP Extracellular Matrix - enzymology Fibroblasts - metabolism Gelatinases - genetics Gene Expression Regulation, Enzymologic - drug effects Glycoproteins - genetics Humans Matrix Metalloproteinase 1 Matrix Metalloproteinase 2 Matrix Metalloproteinase 3 - genetics Metalloendopeptidases - antagonists & inhibitors Metalloendopeptidases - genetics MMP Protease Inhibitors - metabolism Proteins - genetics RNA, Messenger - genetics RNA, Messenger - metabolism Skin - metabolism TIMP Tissue Inhibitor of Metalloproteinase-2 Tissue Inhibitor of Metalloproteinases Up-Regulation - drug effects
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Zusammenfassung:	The influence of dibutyril cyclic AMP (dbcAMP) on the gene expression of matrix metalloproteinases (MMP)-1, MMP-2, MMP-3, tissue inhibitors of metalloproteinases (TIMP)-1 and TIMP-2 was investigated in human skin fibroblasts by northern analyses. The treatment of dbcAMP increased MMP-1 and MMP-3 mRNA levels but decreased TIMP-1 mRNA levels in time and dose dependent manners. Procollagen α1(I), MMP-2 and TIMP-2 mRNA levels were unaltered by this reagent. Our data indicate that dbcAMP potentially enhances the degradation of extracellular components of connective tissue.
ISSN:	0923-1811 1873-569X
DOI:	10.1016/S0923-1811(97)00035-2