Cloning and characterization of an Escherichia coli gene, pcnB, affecting plasmid copy number

Cloning and characterization of an Escherichia coli gene, pcnB, affecting plasmid copy number

A gene, pcnB, affecting the copy number of ColE1-related plasmids has been cloned and mapped to 3.6 min on the Escherichia coli chromosome between panD and fhu. The gene encodes a previously undescribed 48 kD protein. Several independently isolated mutants exhibiting the same phenotype, reduced copy...

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Veröffentlicht in:Molecular microbiology 1989-07, Vol.3 (7), p.903-910
Hauptverfasser: MARCH, J. B, COLLOMS, M. D, HART-DAVIS, D, OLIVER, I. R, MASTERS, M
Format: Artikel
Sprache:eng
Schlagworte:
Alleles
Ampicillin Resistance
Bacterial Proteins - genetics
Bacteriology
Biological and medical sciences
Cloning, Molecular
Escherichia coli
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genes, Bacterial
Genetics
Microbiology
Molecular Weight
Mutation
Phenotype
Plasmids
Protein Biosynthesis
Restriction Mapping
Transcription, Genetic
Transduction, Genetic
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Beschreibung
Zusammenfassung:A gene, pcnB, affecting the copy number of ColE1-related plasmids has been cloned and mapped to 3.6 min on the Escherichia coli chromosome between panD and fhu. The gene encodes a previously undescribed 48 kD protein. Several independently isolated mutants exhibiting the same phenotype, reduced copy number, have been shown to be pcnB-.
ISSN:0950-382X
1365-2958
DOI:10.1111/j.1365-2958.1989.tb00239.x