Functional incorporation of P-glycoprotein into Xenopus oocyte plasma membrane fails to elicit a swelling-evoked conductance
Microinjecton of Xenopus oocytes with P-glycoprotein-containing membranes from multidrug resistant cells following a recently published procedure resulted in the transplantation of the protein to the plasma membrane of the oocytes and was confirmed by Western blot analysis. These oocytes showed a re...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 1997-08, Vol.237 (2), p.407-412 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 412 |
|---|---|
| container_issue | 2 |
| container_start_page | 407 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 237 |
| creator | Aleu, J Ivorra, I Lejarreta, M Gonzalez-Ros, J M Morales, A Ferragut, J A |
| description | Microinjecton of Xenopus oocytes with P-glycoprotein-containing membranes from multidrug resistant cells following a recently published procedure resulted in the transplantation of the protein to the plasma membrane of the oocytes and was confirmed by Western blot analysis. These oocytes showed a reduced intracellular accumulation of daunomycin, when compared to uninjected oocytes or to those injected with membrane vesicles lacking P-glycoprotein, thus indicating that the protein had been incorporated in a transport-competent form. On the other hand, transplantation of P-glycoprotein to the oocyte membrane did not significantly change either the appearance or the properties of swelling-elicited membrane conductance with respect to those determined in oocytes either uninjected or injected with membranes lacking P-glycoprotein. These results do not support a role for P-glycoprotein as a swelling-activated chloride channel. |
| doi_str_mv | 10.1006/bbrc.1997.7150 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_79244198</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79244198</sourcerecordid><originalsourceid>FETCH-LOGICAL-p206t-25a3c45d02edeba3f3bad17289c20f2b471be9e42ff14e3ef8d35b536569a2893</originalsourceid><addsrcrecordid>eNotkL1PwzAQxT2ASimsbEie2FL8laQeUUUBqRIMIHWLbOdcGRI7xA6oEn88RnQ63Xs_nd47hK4oWVJCqlutR7OkUtbLmpbkBM1JVgsm6e4Mncf4TgilopIzNJOsWtVMzNHPZvImueBVh503YRzCqP52HCx-KfbdwYRhDAmcz34KeAc-DFPEIZhDAjx0KvYK99DrUXnAVrku4sxB54xLWOH4DV3n_L6Ar_ABLTbBt5NJyhu4QKdWdREuj3OB3jb3r-vHYvv88LS-2xYDI1UqWKm4EWVLGLSgFbdcq5bWbCUNI5ZpUVMNEgSzlgrgYFctL3XJq7KSKlN8gW7-7-YmnxPE1PQumhwrJw5TbGrJhKBylcHrIzjpHtpmGF2vxkNzfBf_BeGYbkg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79244198</pqid></control><display><type>article</type><title>Functional incorporation of P-glycoprotein into Xenopus oocyte plasma membrane fails to elicit a swelling-evoked conductance</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Aleu, J ; Ivorra, I ; Lejarreta, M ; Gonzalez-Ros, J M ; Morales, A ; Ferragut, J A</creator><creatorcontrib>Aleu, J ; Ivorra, I ; Lejarreta, M ; Gonzalez-Ros, J M ; Morales, A ; Ferragut, J A</creatorcontrib><description>Microinjecton of Xenopus oocytes with P-glycoprotein-containing membranes from multidrug resistant cells following a recently published procedure resulted in the transplantation of the protein to the plasma membrane of the oocytes and was confirmed by Western blot analysis. These oocytes showed a reduced intracellular accumulation of daunomycin, when compared to uninjected oocytes or to those injected with membrane vesicles lacking P-glycoprotein, thus indicating that the protein had been incorporated in a transport-competent form. On the other hand, transplantation of P-glycoprotein to the oocyte membrane did not significantly change either the appearance or the properties of swelling-elicited membrane conductance with respect to those determined in oocytes either uninjected or injected with membranes lacking P-glycoprotein. These results do not support a role for P-glycoprotein as a swelling-activated chloride channel.</description><identifier>ISSN: 0006-291X</identifier><identifier>DOI: 10.1006/bbrc.1997.7150</identifier><identifier>PMID: 9268724</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism ; Cell Line ; Cell Membrane - metabolism ; Drug Resistance, Multiple ; Leukemia L1210 - metabolism ; Leukemia L1210 - pathology ; Membrane Potentials ; Mice ; Oocytes - metabolism ; Oocytes - physiology ; Xenopus</subject><ispartof>Biochemical and biophysical research communications, 1997-08, Vol.237 (2), p.407-412</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9268724$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aleu, J</creatorcontrib><creatorcontrib>Ivorra, I</creatorcontrib><creatorcontrib>Lejarreta, M</creatorcontrib><creatorcontrib>Gonzalez-Ros, J M</creatorcontrib><creatorcontrib>Morales, A</creatorcontrib><creatorcontrib>Ferragut, J A</creatorcontrib><title>Functional incorporation of P-glycoprotein into Xenopus oocyte plasma membrane fails to elicit a swelling-evoked conductance</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Microinjecton of Xenopus oocytes with P-glycoprotein-containing membranes from multidrug resistant cells following a recently published procedure resulted in the transplantation of the protein to the plasma membrane of the oocytes and was confirmed by Western blot analysis. These oocytes showed a reduced intracellular accumulation of daunomycin, when compared to uninjected oocytes or to those injected with membrane vesicles lacking P-glycoprotein, thus indicating that the protein had been incorporated in a transport-competent form. On the other hand, transplantation of P-glycoprotein to the oocyte membrane did not significantly change either the appearance or the properties of swelling-elicited membrane conductance with respect to those determined in oocytes either uninjected or injected with membranes lacking P-glycoprotein. These results do not support a role for P-glycoprotein as a swelling-activated chloride channel.</description><subject>Animals</subject><subject>ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism</subject><subject>Cell Line</subject><subject>Cell Membrane - metabolism</subject><subject>Drug Resistance, Multiple</subject><subject>Leukemia L1210 - metabolism</subject><subject>Leukemia L1210 - pathology</subject><subject>Membrane Potentials</subject><subject>Mice</subject><subject>Oocytes - metabolism</subject><subject>Oocytes - physiology</subject><subject>Xenopus</subject><issn>0006-291X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotkL1PwzAQxT2ASimsbEie2FL8laQeUUUBqRIMIHWLbOdcGRI7xA6oEn88RnQ63Xs_nd47hK4oWVJCqlutR7OkUtbLmpbkBM1JVgsm6e4Mncf4TgilopIzNJOsWtVMzNHPZvImueBVh503YRzCqP52HCx-KfbdwYRhDAmcz34KeAc-DFPEIZhDAjx0KvYK99DrUXnAVrku4sxB54xLWOH4DV3n_L6Ar_ABLTbBt5NJyhu4QKdWdREuj3OB3jb3r-vHYvv88LS-2xYDI1UqWKm4EWVLGLSgFbdcq5bWbCUNI5ZpUVMNEgSzlgrgYFctL3XJq7KSKlN8gW7-7-YmnxPE1PQumhwrJw5TbGrJhKBylcHrIzjpHtpmGF2vxkNzfBf_BeGYbkg</recordid><startdate>19970818</startdate><enddate>19970818</enddate><creator>Aleu, J</creator><creator>Ivorra, I</creator><creator>Lejarreta, M</creator><creator>Gonzalez-Ros, J M</creator><creator>Morales, A</creator><creator>Ferragut, J A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19970818</creationdate><title>Functional incorporation of P-glycoprotein into Xenopus oocyte plasma membrane fails to elicit a swelling-evoked conductance</title><author>Aleu, J ; Ivorra, I ; Lejarreta, M ; Gonzalez-Ros, J M ; Morales, A ; Ferragut, J A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p206t-25a3c45d02edeba3f3bad17289c20f2b471be9e42ff14e3ef8d35b536569a2893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism</topic><topic>Cell Line</topic><topic>Cell Membrane - metabolism</topic><topic>Drug Resistance, Multiple</topic><topic>Leukemia L1210 - metabolism</topic><topic>Leukemia L1210 - pathology</topic><topic>Membrane Potentials</topic><topic>Mice</topic><topic>Oocytes - metabolism</topic><topic>Oocytes - physiology</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aleu, J</creatorcontrib><creatorcontrib>Ivorra, I</creatorcontrib><creatorcontrib>Lejarreta, M</creatorcontrib><creatorcontrib>Gonzalez-Ros, J M</creatorcontrib><creatorcontrib>Morales, A</creatorcontrib><creatorcontrib>Ferragut, J A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aleu, J</au><au>Ivorra, I</au><au>Lejarreta, M</au><au>Gonzalez-Ros, J M</au><au>Morales, A</au><au>Ferragut, J A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional incorporation of P-glycoprotein into Xenopus oocyte plasma membrane fails to elicit a swelling-evoked conductance</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1997-08-18</date><risdate>1997</risdate><volume>237</volume><issue>2</issue><spage>407</spage><epage>412</epage><pages>407-412</pages><issn>0006-291X</issn><abstract>Microinjecton of Xenopus oocytes with P-glycoprotein-containing membranes from multidrug resistant cells following a recently published procedure resulted in the transplantation of the protein to the plasma membrane of the oocytes and was confirmed by Western blot analysis. These oocytes showed a reduced intracellular accumulation of daunomycin, when compared to uninjected oocytes or to those injected with membrane vesicles lacking P-glycoprotein, thus indicating that the protein had been incorporated in a transport-competent form. On the other hand, transplantation of P-glycoprotein to the oocyte membrane did not significantly change either the appearance or the properties of swelling-elicited membrane conductance with respect to those determined in oocytes either uninjected or injected with membranes lacking P-glycoprotein. These results do not support a role for P-glycoprotein as a swelling-activated chloride channel.</abstract><cop>United States</cop><pmid>9268724</pmid><doi>10.1006/bbrc.1997.7150</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1997-08, Vol.237 (2), p.407-412 |
| issn | 0006-291X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79244198 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism Cell Line Cell Membrane - metabolism Drug Resistance, Multiple Leukemia L1210 - metabolism Leukemia L1210 - pathology Membrane Potentials Mice Oocytes - metabolism Oocytes - physiology Xenopus |
| title | Functional incorporation of P-glycoprotein into Xenopus oocyte plasma membrane fails to elicit a swelling-evoked conductance |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T00%3A18%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Functional%20incorporation%20of%20P-glycoprotein%20into%20Xenopus%20oocyte%20plasma%20membrane%20fails%20to%20elicit%20a%20swelling-evoked%20conductance&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Aleu,%20J&rft.date=1997-08-18&rft.volume=237&rft.issue=2&rft.spage=407&rft.epage=412&rft.pages=407-412&rft.issn=0006-291X&rft_id=info:doi/10.1006/bbrc.1997.7150&rft_dat=%3Cproquest_pubme%3E79244198%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=79244198&rft_id=info:pmid/9268724&rfr_iscdi=true |