Functional incorporation of P-glycoprotein into Xenopus oocyte plasma membrane fails to elicit a swelling-evoked conductance
Microinjecton of Xenopus oocytes with P-glycoprotein-containing membranes from multidrug resistant cells following a recently published procedure resulted in the transplantation of the protein to the plasma membrane of the oocytes and was confirmed by Western blot analysis. These oocytes showed a re...
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Veröffentlicht in: | Biochemical and biophysical research communications 1997-08, Vol.237 (2), p.407-412 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Microinjecton of Xenopus oocytes with P-glycoprotein-containing membranes from multidrug resistant cells following a recently published procedure resulted in the transplantation of the protein to the plasma membrane of the oocytes and was confirmed by Western blot analysis. These oocytes showed a reduced intracellular accumulation of daunomycin, when compared to uninjected oocytes or to those injected with membrane vesicles lacking P-glycoprotein, thus indicating that the protein had been incorporated in a transport-competent form. On the other hand, transplantation of P-glycoprotein to the oocyte membrane did not significantly change either the appearance or the properties of swelling-elicited membrane conductance with respect to those determined in oocytes either uninjected or injected with membranes lacking P-glycoprotein. These results do not support a role for P-glycoprotein as a swelling-activated chloride channel. |
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ISSN: | 0006-291X |
DOI: | 10.1006/bbrc.1997.7150 |