Amino acid sequence of the crustacean hyperglycemic hormone (CHH) from the shore crab, Carcinus maenas

Crustacean hyperglycemic hormone (CHH) was isolated from sinus glands of Carcinus maenas, and its primary structure was determined by manual microsequencing, using the DABITC-PITC double-coupling method. The neurohormone consists of 72 amino acid residues (8524 Da). Three disulfide bridges are prese...

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Veröffentlicht in:	FEBS letters 1989-09, Vol.255 (1), p.10-14
Hauptverfasser:	Kegel, Günter, Reichwein, Barbara, Weese, Susanne, Gaus, Gabriele, Peter-Kataliníc, Jasna, Keller, Rainer
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acids Amino Acids - analysis Aminoacids, peptides. Hormones. Neuropeptides Aminopeptidases Analytical, structural and metabolic biochemistry Animals Arthropod Proteins biochemical composition Biological and medical sciences Brachyura Carboxypeptidases Carcinus maenas Chromatography, High Pressure Liquid Crustacean hyperglycemic hormone DABITC, 4-N,N-dimethylaminoazobenzene 4′-isothiocyanate DABTH, 4-N,N-dimethylaminoazobenzene thiohydantoin Disulfides - analysis Fundamental and applied biological sciences. Psychology hormones HPLC, high-performance liquid chromatography Invertebrate Hormones - analysis Invertebrate Hormones - isolation & purification Marine Mass Spectrometry Molecular Sequence Data molecular structure Nerve Tissue Proteins - analysis Nerve Tissue Proteins - isolation & purification Neuropeptide Peptide Fragments - analysis PITC, phenylisothiocyanate Proteins Serine Endopeptidases TFA, trifluoroacetic acid
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Zusammenfassung:	Crustacean hyperglycemic hormone (CHH) was isolated from sinus glands of Carcinus maenas, and its primary structure was determined by manual microsequencing, using the DABITC-PITC double-coupling method. The neurohormone consists of 72 amino acid residues (8524 Da). Three disulfide bridges are present and both the N- and C-terminus are blocked. CHH does not show significant sequence homology to any known peptide hormone or protein.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(89)81051-8