Increased level of apolipoprotein B mRNA in the liver of ventromedial hypothalamus lesioned obese rats

The mRNA level of apolipoprotein B (apoB), which is a principal protein component of nascent very low density lipoprotein (VLDL), was determined in parallel with the measurement of acetyl-coenzyme A (Ac-CoA) car☐ylase activity in the liver of ventromedial hypothalamus (VMH) lesioned obese rats. Eigh...

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Veröffentlicht in:	Biochemical and biophysical research communications 1989-09, Vol.163 (2), p.1107-1112
Hauptverfasser:	Inui, Yoshiaki, Kawata, Sumio, Matsuzawa, Yuji, Tokunaga, Katsuto, Fujioka, Shigenori, Tamura, Shinji, Kobatake, Takashi, Keno, Yoshiaki, Tarui, Seiichiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins - metabolism Animals Apolipoproteins B - metabolism ARN Biological and medical sciences Blotting, Northern Body Weight BRAIN CEREBRO ENCEPHALE Female HYPOTHALAMIC LESIONS LESION LESIONES LESIONS LIPOPROTEINAS LIPOPROTEINE LIPOPROTEINS Lipoproteins, VLDL - metabolism Liver - metabolism Medical sciences Metabolic diseases Obesity Obesity - metabolism OVERWEIGHT RAT RATA RATS Rats, Inbred Strains RNA RNA, Messenger - metabolism SOBREPESO SURPOIDS Ventromedial Hypothalamic Nucleus - pathology VERY LOW DENSITY LIPOPROTEIN
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Zusammenfassung:	The mRNA level of apolipoprotein B (apoB), which is a principal protein component of nascent very low density lipoprotein (VLDL), was determined in parallel with the measurement of acetyl-coenzyme A (Ac-CoA) car☐ylase activity in the liver of ventromedial hypothalamus (VMH) lesioned obese rats. Eight weeks after the electrolysis of the bilateral VMH, the level of apoB mRNA in the VMH-lesioned rats was about 1.5-fold higher than that in the sham-operated rats, indicating increased apoB synthesis in the liver of the VMH-lesioned obese rats. The activity of Ac-CoA car☐ylase, which is a rate-limiting enzyme for the fatty acid biosynthesis, was about 1.8-fold higher in the VMH-lesioned rats. These observations indicated that VLDL synthesis is increased in the liver of VMH-lesioned obese rats.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(89)92335-8