Identification of a type 1 insulin-like growth factor binding protein (IGF BP) in serum from rats with diabetes mellitus

Circulating insulin-like growth factor binding protein (IGF BP) activity is increased in animals with streptozotocin-induced diabetes. Separation of BPs by SDS/PAGE for ligand and immunoblot analysis revealed that a 32,000 molecular weight BP is present and increased in diabetic serum. This BP is im...

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Veröffentlicht in:	Biochemical and biophysical research communications 1989-09, Vol.163 (2), p.882-887
Hauptverfasser:	Unterman, Terry G., Oehler, David T., Becker, Ronald E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Labels Analytical, structural and metabolic biochemistry Animals Binding and carrier proteins Biological and medical sciences Blotting, Western Carrier Proteins - isolation & purification diabetes mellitus Diabetes Mellitus, Experimental - blood Electrophoresis, Polyacrylamide Gel Female Fundamental and applied biological sciences. Psychology Insulin-Like Growth Factor Binding Proteins Insulin-Like Growth Factor I - blood Insulin-Like Growth Factor I - isolation & purification Precipitin Tests Proteins Rats Rats, Inbred Strains Somatomedins - blood Somatomedins - isolation & purification
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Beschreibung
Zusammenfassung:	Circulating insulin-like growth factor binding protein (IGF BP) activity is increased in animals with streptozotocin-induced diabetes. Separation of BPs by SDS/PAGE for ligand and immunoblot analysis revealed that a 32,000 molecular weight BP is present and increased in diabetic serum. This BP is immunologically distinct from the low molecular weight fetal rat BP (rBP2) and is related to the human amniotic fluid BP (hBP1) that is increased in patients with insulin dependent diabetes mellitus.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(89)92304-8