The Ligand-binding Site of Buspirone Analogues at the 5-HT1A Receptor

A three‐dimensional model of the 5‐HT1A receptor in man was constructed by molecular‐modelling techniques and used to study the molecular interactions of a series of buspirone analogues with the 5‐HT1A receptor by molecular‐mechanical‐energy minimization and molecular‐dynamics simulations. The receptor has seven trans‐membrane α helices (TMHs) organized according to the electron‐density‐projection map of visual rhodopsin, and includes all loops between TMHs and the N‐ and C‐terminal parts. The best fit between the buspirone analogues and the receptor model was obtained with the quinolinyl part of the ligand molecules interacting with amino acids in TMH6, the imide group interacting with amino acids in TMH2, TMH3 and TMH7, and the carbonyl groups hydrogen‐bonded with Ser86 and Ser393. The ligand‐binding rank order deduced from the experimentally determined inhibition constant was reproduced by calculation of receptor‐binding energies of the buspirone analogues. The models suggest that steric hindrance and repulsive forces between the receptor and the imide group of the buspirone analogues are the most important determinants of ligand‐binding affinity for discriminating between these ligands.