Monoaminergic and gabaergic terminations in phrenic nucleus of rat identified by immunohistochemical labeling
The termination patterns of axons in the phrenic nucleus immunoreactive to synthetic enzymes for catecholamines and for serotonin and GABA were studied in rats. Spinal cord tissue in which phrenic motoneurons were retrogradely labeled with horseradish peroxidase was incubated with antisera against d...
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| Veröffentlicht in: | Neuroscience 1989, Vol.31 (1), p.105-113 |
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| creator | Zhan, W.-Z. Ellenberger, H.H. Feldman, J.L. |
| description | The termination patterns of axons in the phrenic nucleus immunoreactive to synthetic enzymes for catecholamines and for serotonin and GABA were studied in rats. Spinal cord tissue in which phrenic motoneurons were retrogradely labeled with horseradish peroxidase was incubated with antisera against dopamine β-hydroxylase, phenylethanolamine-
N-methyltransferase, 5-hydroxytryptamine, and GABA to identify presumptive terminations of monoaminergic and GABAergic neurons onto identified phrenic motoneurons. In the C3 to C5 spinal cord, 5-hydroxytryptamine-, dopamine β-hydroxylase- and GABA-like positive terminals with varicosities formed a dense network, with presumptive synaptic contacts on dendrites and somas of phrenic motoneurons. A similar pattern of terminations was also observed in adjacent (non-respiratory muscle) motoneuron pools. There were fewer phenylethanolamine-
N-methyltransferase-positive terminal arborizations in the cervical spinal cord compared to thoracic spinal cord; phenylethanolamine-
N-methyltransferase terminals were not seen in the vicinity of phrenic motoneurons.
These results suggest that phrenic motoneuronal activity is influenced by multiple supraspinal inputs utilizing different neurotransmitters. These transmitters also mediate inputs to other (nearby) spinal motoneurons and thus are not unique for signal transmission to phrenic motoneurons. |
| doi_str_mv | 10.1016/0306-4522(89)90033-X |
| format | Article |
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N-methyltransferase, 5-hydroxytryptamine, and GABA to identify presumptive terminations of monoaminergic and GABAergic neurons onto identified phrenic motoneurons. In the C3 to C5 spinal cord, 5-hydroxytryptamine-, dopamine β-hydroxylase- and GABA-like positive terminals with varicosities formed a dense network, with presumptive synaptic contacts on dendrites and somas of phrenic motoneurons. A similar pattern of terminations was also observed in adjacent (non-respiratory muscle) motoneuron pools. There were fewer phenylethanolamine-
N-methyltransferase-positive terminal arborizations in the cervical spinal cord compared to thoracic spinal cord; phenylethanolamine-
N-methyltransferase terminals were not seen in the vicinity of phrenic motoneurons.
These results suggest that phrenic motoneuronal activity is influenced by multiple supraspinal inputs utilizing different neurotransmitters. These transmitters also mediate inputs to other (nearby) spinal motoneurons and thus are not unique for signal transmission to phrenic motoneurons.</description><identifier>ISSN: 0306-4522</identifier><identifier>EISSN: 1873-7544</identifier><identifier>DOI: 10.1016/0306-4522(89)90033-X</identifier><identifier>PMID: 2771052</identifier><identifier>CODEN: NRSCDN</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Biogenic Monoamines - metabolism ; Biological and medical sciences ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Dopamine beta-Hydroxylase - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; gamma-Aminobutyric Acid - metabolism ; Immunohistochemistry ; Male ; Motor Neurons - cytology ; Motor Neurons - metabolism ; Nerve Endings - metabolism ; Nerve Endings - ultrastructure ; Phenylethanolamine N-Methyltransferase - metabolism ; Phrenic Nerve - cytology ; Phrenic Nerve - metabolism ; Rats ; Rats, Inbred Strains ; Serotonin - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience, 1989, Vol.31 (1), p.105-113</ispartof><rights>1989 IBRO</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-ce4fd0e3ffde89c528dddfb7f02190dfa64daf1925327fdb898a7b7d389cde6d3</citedby><cites>FETCH-LOGICAL-c417t-ce4fd0e3ffde89c528dddfb7f02190dfa64daf1925327fdb898a7b7d389cde6d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0306-4522(89)90033-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6807127$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2771052$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhan, W.-Z.</creatorcontrib><creatorcontrib>Ellenberger, H.H.</creatorcontrib><creatorcontrib>Feldman, J.L.</creatorcontrib><title>Monoaminergic and gabaergic terminations in phrenic nucleus of rat identified by immunohistochemical labeling</title><title>Neuroscience</title><addtitle>Neuroscience</addtitle><description>The termination patterns of axons in the phrenic nucleus immunoreactive to synthetic enzymes for catecholamines and for serotonin and GABA were studied in rats. Spinal cord tissue in which phrenic motoneurons were retrogradely labeled with horseradish peroxidase was incubated with antisera against dopamine β-hydroxylase, phenylethanolamine-
N-methyltransferase, 5-hydroxytryptamine, and GABA to identify presumptive terminations of monoaminergic and GABAergic neurons onto identified phrenic motoneurons. In the C3 to C5 spinal cord, 5-hydroxytryptamine-, dopamine β-hydroxylase- and GABA-like positive terminals with varicosities formed a dense network, with presumptive synaptic contacts on dendrites and somas of phrenic motoneurons. A similar pattern of terminations was also observed in adjacent (non-respiratory muscle) motoneuron pools. There were fewer phenylethanolamine-
N-methyltransferase-positive terminal arborizations in the cervical spinal cord compared to thoracic spinal cord; phenylethanolamine-
N-methyltransferase terminals were not seen in the vicinity of phrenic motoneurons.
These results suggest that phrenic motoneuronal activity is influenced by multiple supraspinal inputs utilizing different neurotransmitters. These transmitters also mediate inputs to other (nearby) spinal motoneurons and thus are not unique for signal transmission to phrenic motoneurons.</description><subject>Animals</subject><subject>Biogenic Monoamines - metabolism</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Dopamine beta-Hydroxylase - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma-Aminobutyric Acid - metabolism</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Motor Neurons - cytology</subject><subject>Motor Neurons - metabolism</subject><subject>Nerve Endings - metabolism</subject><subject>Nerve Endings - ultrastructure</subject><subject>Phenylethanolamine N-Methyltransferase - metabolism</subject><subject>Phrenic Nerve - cytology</subject><subject>Phrenic Nerve - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Serotonin - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0306-4522</issn><issn>1873-7544</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo6-zqP1DIQWQ9tOaju9O5LMjiF6x4UdhbSCeVmZLuZEy6hf33ZpxhjlqXUHmfeineIuQFZ2854_07JlnftJ0Q14N-oxmTsrl_RDZ8ULJRXds-Jpsz8pRclvKT1epaeUEuhFKcdWJD5q8pJjtjhLxFR230dGtHe-wWyFWxC6ZYKEa632WI9T-uboK10BRotgtFD3HBgODp-EBxnteYdliW5HYwo7MTnewIE8btM_Ik2KnA89N7RX58_PD99nNz9-3Tl9v3d41ruVoaB23wDGQIHgbtOjF478OoAhNcMx9s33obuBadFCr4cdCDVaPyssIeei-vyOuj7z6nXyuUxcxYHEyTjZDWYpTmauiU_i_Iu-qpFa9gewRdTqVkCGafcbb5wXBmDucwh6zNIWszaPP3HOa-jr08-a_jDP48dMq_6q9Oui01qJBtdFjOWD8wxYWq2M0Rgxrab4RsikOIDjxmcIvxCf-9xx8he6mt</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>Zhan, W.-Z.</creator><creator>Ellenberger, H.H.</creator><creator>Feldman, J.L.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>1989</creationdate><title>Monoaminergic and gabaergic terminations in phrenic nucleus of rat identified by immunohistochemical labeling</title><author>Zhan, W.-Z. ; Ellenberger, H.H. ; Feldman, J.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-ce4fd0e3ffde89c528dddfb7f02190dfa64daf1925327fdb898a7b7d389cde6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Biogenic Monoamines - metabolism</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Dopamine beta-Hydroxylase - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma-Aminobutyric Acid - metabolism</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Motor Neurons - cytology</topic><topic>Motor Neurons - metabolism</topic><topic>Nerve Endings - metabolism</topic><topic>Nerve Endings - ultrastructure</topic><topic>Phenylethanolamine N-Methyltransferase - metabolism</topic><topic>Phrenic Nerve - cytology</topic><topic>Phrenic Nerve - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Serotonin - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhan, W.-Z.</creatorcontrib><creatorcontrib>Ellenberger, H.H.</creatorcontrib><creatorcontrib>Feldman, J.L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhan, W.-Z.</au><au>Ellenberger, H.H.</au><au>Feldman, J.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monoaminergic and gabaergic terminations in phrenic nucleus of rat identified by immunohistochemical labeling</atitle><jtitle>Neuroscience</jtitle><addtitle>Neuroscience</addtitle><date>1989</date><risdate>1989</risdate><volume>31</volume><issue>1</issue><spage>105</spage><epage>113</epage><pages>105-113</pages><issn>0306-4522</issn><eissn>1873-7544</eissn><coden>NRSCDN</coden><abstract>The termination patterns of axons in the phrenic nucleus immunoreactive to synthetic enzymes for catecholamines and for serotonin and GABA were studied in rats. Spinal cord tissue in which phrenic motoneurons were retrogradely labeled with horseradish peroxidase was incubated with antisera against dopamine β-hydroxylase, phenylethanolamine-
N-methyltransferase, 5-hydroxytryptamine, and GABA to identify presumptive terminations of monoaminergic and GABAergic neurons onto identified phrenic motoneurons. In the C3 to C5 spinal cord, 5-hydroxytryptamine-, dopamine β-hydroxylase- and GABA-like positive terminals with varicosities formed a dense network, with presumptive synaptic contacts on dendrites and somas of phrenic motoneurons. A similar pattern of terminations was also observed in adjacent (non-respiratory muscle) motoneuron pools. There were fewer phenylethanolamine-
N-methyltransferase-positive terminal arborizations in the cervical spinal cord compared to thoracic spinal cord; phenylethanolamine-
N-methyltransferase terminals were not seen in the vicinity of phrenic motoneurons.
These results suggest that phrenic motoneuronal activity is influenced by multiple supraspinal inputs utilizing different neurotransmitters. These transmitters also mediate inputs to other (nearby) spinal motoneurons and thus are not unique for signal transmission to phrenic motoneurons.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>2771052</pmid><doi>10.1016/0306-4522(89)90033-X</doi><tpages>9</tpages></addata></record> |
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| ispartof | Neuroscience, 1989, Vol.31 (1), p.105-113 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biogenic Monoamines - metabolism Biological and medical sciences Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Dopamine beta-Hydroxylase - metabolism Female Fundamental and applied biological sciences. Psychology gamma-Aminobutyric Acid - metabolism Immunohistochemistry Male Motor Neurons - cytology Motor Neurons - metabolism Nerve Endings - metabolism Nerve Endings - ultrastructure Phenylethanolamine N-Methyltransferase - metabolism Phrenic Nerve - cytology Phrenic Nerve - metabolism Rats Rats, Inbred Strains Serotonin - metabolism Vertebrates: nervous system and sense organs |
| title | Monoaminergic and gabaergic terminations in phrenic nucleus of rat identified by immunohistochemical labeling |
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