Enhanced Chemiluminescent Assay for Measuring the Total Antioxidant Capacity of Serum, Saliva and Crevicular Fluid

This paper reports the development of an enhanced chemiluminescent (ECL) assay for measuring the total antioxidant (AO) capacity of serum, saliva and a fluid collectable from the gum margin called gingival crevicular fluid (GCF). The theory behind the assay is explained, and the optimum conditions f...

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Veröffentlicht in:	Annals of clinical biochemistry 1997-07, Vol.34 (4), p.412-421
Hauptverfasser:	Chapple, I L C, Mason, G I, Garner, I, Matthews, J B, Thorpe, G H, Maxwell, S R J, Whitehead, T P
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Antioxidants - analysis Biological and medical sciences Blood Chemical Analysis Calibration Chromans - analysis Cryopreservation Female Gingival Crevicular Fluid - chemistry Horseradish Peroxidase - metabolism Humans Investigative techniques, diagnostic techniques (general aspects) Kinetics Luminescent Measurements Male Medical sciences Miscellaneous. Technology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Periodontitis - metabolism Reactive Oxygen Species - metabolism Saliva - chemistry Vitamin E - analysis
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Zusammenfassung:	This paper reports the development of an enhanced chemiluminescent (ECL) assay for measuring the total antioxidant (AO) capacity of serum, saliva and a fluid collectable from the gum margin called gingival crevicular fluid (GCF). The theory behind the assay is explained, and the optimum conditions for the assay, and for storage of reagents and clinical samples is described. Calibration lines were linear (R≥0·99; P
ISSN:	0004-5632 1758-1001
DOI:	10.1177/000456329703400413