Optimization of the synthesis of peptide combinatorial libraries using a one-pot method

Conditions for the synthesis of synthetic peptide combinatorial libraries (SPCLs) from mixtures of amino acids were explored. In a one-pot synthesis, the effect of the starting concentrations of amino acids on the resulting library composition was studied, and the optimum balance of amino acids was...

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Veröffentlicht in:Molecular diversity 1997, Vol.2 (3), p.147-155
Hauptverfasser: Herman, L W, Tarr, G, Kates, S A
Format: Artikel
Sprache:eng
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Zusammenfassung:Conditions for the synthesis of synthetic peptide combinatorial libraries (SPCLs) from mixtures of amino acids were explored. In a one-pot synthesis, the effect of the starting concentrations of amino acids on the resulting library composition was studied, and the optimum balance of amino acids was determined. Protein sequencing, MALDI-TOF, and amino acid analysis were used for the evaluation of the libraries, and their relative merits-are discussed. The effects of continuous-flow automated synthesis instrumentation in conjunction with polyethylene glycol-polystyrene (PEG-PS) graft supports and various cleavage cocktails on the successful synthesis of SPCLs were examined.
ISSN:1381-1991
1573-501X
DOI:10.1007/BF01682202