In Vitro Pemphigus Vulgaris Model Using Organotypic Cultures of Human Epidermal Keratinocytes

Using the Combi-ring-dish (CRD), a new culture device, organotypic cultures of human epidermal keratinocytes were grown on bovine eye lens capsules. In these highly differentiated cultures, typical suprabasal acantholysis was induced by pemphigus vulgaris antibodies. This in vitro pemphigus vulgaris...

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Veröffentlicht in:	Journal of investigative dermatology 1989-08, Vol.93 (2), p.263-267
Hauptverfasser:	Hunziker, Thomas, Boillat, Colette, Gerber, Heinz A, Wiesmann, Utrich, Wintroub, Bruce U
Format:	Artikel
Sprache:	eng
Schlagworte:	Acantholysis - immunology Acantholysis - pathology Animals Antibodies Antibodies - immunology Biological and medical sciences Bullous diseases of the skin Cattle Cells, Cultured Cytological Techniques - instrumentation Dermatology Epidermis - pathology Epidermis - ultrastructure Eye lens Humans Keratinocytes Keratins Medical sciences Microscopy, Electron Pemphigus - immunology Pemphigus - pathology pemphigus vulgaris
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Beschreibung
Zusammenfassung:	Using the Combi-ring-dish (CRD), a new culture device, organotypic cultures of human epidermal keratinocytes were grown on bovine eye lens capsules. In these highly differentiated cultures, typical suprabasal acantholysis was induced by pemphigus vulgaris antibodies. This in vitro pemphigus vulgaris model may be used to analyse keratinocyte-derived factors causing acantholysis in experimental pemphigus.
ISSN:	0022-202X 1523-1747
DOI:	10.1111/1523-1747.ep12277586