Participation of the hup gene product in site-specific DNA inversion in Escherichia coli
The closely related Escherichia coli genes hupA and hupB each encode a bacterial histone-like protein HU. We report here that DNA inversion mediated by hin, gin, pin and rci but not by cin is blocked in a hupA hupB double mutant, although inversions in these systems occur in the hupA or hupB single...
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Veröffentlicht in: | Gene 1989, Vol.76 (2), p.345-352 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The closely related
Escherichia coli genes
hupA and
hupB each encode a bacterial histone-like protein HU. We report here that DNA inversion mediated by
hin, gin, pin and
rci but not by
cin is blocked in a
hupA hupB double mutant, although inversions in these systems occur in the
hupA or
hupB single mutant as efficiently as in the wild-type strain. These findings show that HU protein participates in site-specific DNA inversion in
E. coli and that only one subunit, either HU-1 or HU-2, is sufficient for this inversion. |
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ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/0378-1119(89)90174-1 |