An automated high-performance liquid chromatographic trace enrichment method for the determination of metoclopramide in serum and its application to a bioequivalence human volunteer study

An automated high-performance liquid chromatographic trace enrichment method for the determination of metoclopramide in serum and its application to a bioequivalence human volunteer study

An automated trace enrichment method for metoclopramide is described. Serum was injected on to a short column packed with PLRP-S 100A (a polymeric reversed-phase material). The unwanted components were washed off with borate buffer (pH 9.8) before switching to a Spherisorb ODS column for the separat...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Food and chemical toxicology 1989, Vol.27 (5), p.341-345
Hauptverfasser: Fairhead, A.P., Brooks, S.G., Butterworth, K.R., Mangham, B.A.
Format: Artikel
Sprache:eng
Schlagworte:
Adult
Analysis
Biological and medical sciences
Biological Availability
Chromatography, High Pressure Liquid - methods
Female
General pharmacology
Humans
Male
Medical sciences
Metoclopramide - blood
Metoclopramide - pharmacokinetics
Pharmacology. Drug treatments
Predictive Value of Tests
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:An automated trace enrichment method for metoclopramide is described. Serum was injected on to a short column packed with PLRP-S 100A (a polymeric reversed-phase material). The unwanted components were washed off with borate buffer (pH 9.8) before switching to a Spherisorb ODS column for the separation. The limit of detection in serum samples was 2 ng/ml. The method was used in a pharmacokinetic study to compare the bioavailability of two formulations of the drug.
ISSN:0278-6915
1873-6351
DOI:10.1016/0278-6915(89)90137-3