CHO transfectants produce large amounts of recombinant protein in suspension culture

Chinese hamster ovary (CHO) cells transfected with various genes are widely used as adherent cell monolayers to produce recombinant proteins. In this report we present a new culture technique for CHO cells transfected with the vector pPOL-DHFR-CD14 using a minifermenter (miniPERM, Heraeus) for the p...

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Veröffentlicht in:	Journal of immunological methods 1997-05, Vol.204 (1), p.99-102
Hauptverfasser:	Schütt, Christine, Fürll, Birgitt, Stelter, Felix, Jack, Robert S, Witt, Sabine
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Culture Techniques CHO Cells - metabolism CHO transfectant Cloning, Molecular - methods Cricetinae Humans Lipopolysaccharide Receptors - biosynthesis Lipopolysaccharide Receptors - genetics Minifermenter Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Spheroid Suspension culture Transfection
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Zusammenfassung:	Chinese hamster ovary (CHO) cells transfected with various genes are widely used as adherent cell monolayers to produce recombinant proteins. In this report we present a new culture technique for CHO cells transfected with the vector pPOL-DHFR-CD14 using a minifermenter (miniPERM, Heraeus) for the production of recombinant human endotoxin receptor CD14 (rCD14). The transfectants were cultured for 12–17 days under serum-free conditions and formed spheroids. From this system we harvested supernatants containing up to 3.1 mg/ml recombinant CD14 (rCD14). This represents a 200-fold increase of rCD14 yield compared to conventional adherent CHO cell culture.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/S0022-1759(97)00027-6