Thyroid hormone modulation of TRH precursor levels in rat hypothalamus, pituitary, thyroid and blood
In the present study we have examined the in vivo effects of thyroid hormones and TRH on tissue and blood levels of TRH and TRH-Gly (pGlu-His-Pro-Gly), a TRH precursor. Using specific radioimmunoassays (RIAs), we measured TRH immunoreactivity (TRH-IR) and TRH-Gly-IR concentrations in blood, hypothal...
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| Veröffentlicht in: | Peptides (New York, N.Y. : 1980) N.Y. : 1980), 1989, Vol.10 (1), p.145-155 |
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| creator | Simard, Marie Pekary, A.Eugene Smith, Vierka P. Hershman, Jerome M. |
| description | In the present study we have examined the in vivo effects of thyroid hormones and TRH on tissue and blood levels of TRH and TRH-Gly (pGlu-His-Pro-Gly), a TRH precursor. Using specific radioimmunoassays (RIAs), we measured TRH immunoreactivity (TRH-IR) and TRH-Gly-IR concentrations in blood, hypothalamus, anterior and posterior pituitary, and thyroid in euthyroid, hypothyroid and thyroxine (T4)-treated 250 g male Sprague-Dawley rats. TRH-Gly-IR and TRH-IR were detected in all of these tissues. Highly significant positive correlations between whole blood TRH-Gly-IR levels and the corresponding serum TSH values (
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| doi_str_mv | 10.1016/0196-9781(89)90091-0 |
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p<0.01), whole blood TRH-IR versus serum TSH (
p<0.01) and whole blood TRH-Gly-IR versus whole blood TRH-IR (
p<0.01) are consistent with cosecretion of TRH and TRH precursor peptides into the circulation. Euthyroid rats injected with TRH IP (1 μg/100 g b.wt.) and hypothyroid rats had 4-fold higher whole blood TRH-Gly-IR levels compared to euthyroid controls (
p<0.0005). Injection of TRH into euthyroid rats significantly increased the TRH-Gly-IR concentration in the hypothalamus, anterior and posterior pituitary and thyroid. The increase in blood TRH-Gly-IR following intravenous TRH may be due, in part, to partial saturation of TRH-degrading enzymes in blood and cell membranes. The ratio of TRH-Gly to TRH was significantly increased in the anterior pituitary by hypothyroidism and TRH injection, suggesting that thyroid hormones and TRH regulate the alpha-amidation of TRH-Gly to form TRH in this tissue. TRH-Gly levels of pooled pituitary and thyroid extracts quantitated by a combination of TRH-Gly RIA and high performance liquid chromatography (HPLC) revealed several-fold increases following incubation at 60°C. Heating at this temperature may block the alpha-amidation activity in extra-hypothalamic tissues but not the “trypsin-like” enzymes which cleave prepro-TRH into TRH-Gly-immunoreactive peptides.</description><identifier>ISSN: 0196-9781</identifier><identifier>EISSN: 1873-5169</identifier><identifier>DOI: 10.1016/0196-9781(89)90091-0</identifier><identifier>PMID: 2501768</identifier><identifier>CODEN: PPTDD5</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Blood ; Chromatography ; Fundamental and applied biological sciences. Psychology ; General aspects. Hormone interactions. Hormone actions on several organ systems. Adaptive reactions ; Hypothalamus ; Hypothalamus - drug effects ; Hypothalamus - metabolism ; Hypothyroidism - blood ; Male ; Pituitary ; Pituitary Gland - drug effects ; Pituitary Gland - metabolism ; Pyrrolidonecarboxylic Acid - analogs & derivatives ; Radioimmunoassay ; Rats ; Rats, Inbred Strains ; Reference Values ; Thyroid ; Thyroid Gland - drug effects ; Thyroid Gland - metabolism ; Thyroidectomy ; Thyrotropin - blood ; Thyrotropin-releasing hormone ; Thyrotropin-Releasing Hormone - analogs & derivatives ; Thyrotropin-Releasing Hormone - blood ; Thyrotropin-Releasing Hormone - metabolism ; Thyrotropin-Releasing Hormone - pharmacology ; Thyroxine - blood ; Thyroxine - pharmacology ; Triiodothyronine - blood ; Triiodothyronine - pharmacology ; Vertebrates: endocrinology</subject><ispartof>Peptides (New York, N.Y. : 1980), 1989, Vol.10 (1), p.145-155</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-b85c850478eb7ebfc69fc7943e38e3b6a9f73562f530ed472874f3b30a92e1773</citedby><cites>FETCH-LOGICAL-c417t-b85c850478eb7ebfc69fc7943e38e3b6a9f73562f530ed472874f3b30a92e1773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0196-9781(89)90091-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6768769$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2501768$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Simard, Marie</creatorcontrib><creatorcontrib>Pekary, A.Eugene</creatorcontrib><creatorcontrib>Smith, Vierka P.</creatorcontrib><creatorcontrib>Hershman, Jerome M.</creatorcontrib><title>Thyroid hormone modulation of TRH precursor levels in rat hypothalamus, pituitary, thyroid and blood</title><title>Peptides (New York, N.Y. : 1980)</title><addtitle>Peptides</addtitle><description>In the present study we have examined the in vivo effects of thyroid hormones and TRH on tissue and blood levels of TRH and TRH-Gly (pGlu-His-Pro-Gly), a TRH precursor. Using specific radioimmunoassays (RIAs), we measured TRH immunoreactivity (TRH-IR) and TRH-Gly-IR concentrations in blood, hypothalamus, anterior and posterior pituitary, and thyroid in euthyroid, hypothyroid and thyroxine (T4)-treated 250 g male Sprague-Dawley rats. TRH-Gly-IR and TRH-IR were detected in all of these tissues. Highly significant positive correlations between whole blood TRH-Gly-IR levels and the corresponding serum TSH values (
p<0.01), whole blood TRH-IR versus serum TSH (
p<0.01) and whole blood TRH-Gly-IR versus whole blood TRH-IR (
p<0.01) are consistent with cosecretion of TRH and TRH precursor peptides into the circulation. Euthyroid rats injected with TRH IP (1 μg/100 g b.wt.) and hypothyroid rats had 4-fold higher whole blood TRH-Gly-IR levels compared to euthyroid controls (
p<0.0005). Injection of TRH into euthyroid rats significantly increased the TRH-Gly-IR concentration in the hypothalamus, anterior and posterior pituitary and thyroid. The increase in blood TRH-Gly-IR following intravenous TRH may be due, in part, to partial saturation of TRH-degrading enzymes in blood and cell membranes. The ratio of TRH-Gly to TRH was significantly increased in the anterior pituitary by hypothyroidism and TRH injection, suggesting that thyroid hormones and TRH regulate the alpha-amidation of TRH-Gly to form TRH in this tissue. TRH-Gly levels of pooled pituitary and thyroid extracts quantitated by a combination of TRH-Gly RIA and high performance liquid chromatography (HPLC) revealed several-fold increases following incubation at 60°C. Heating at this temperature may block the alpha-amidation activity in extra-hypothalamic tissues but not the “trypsin-like” enzymes which cleave prepro-TRH into TRH-Gly-immunoreactive peptides.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Chromatography</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. Hormone interactions. Hormone actions on several organ systems. Adaptive reactions</subject><subject>Hypothalamus</subject><subject>Hypothalamus - drug effects</subject><subject>Hypothalamus - metabolism</subject><subject>Hypothyroidism - blood</subject><subject>Male</subject><subject>Pituitary</subject><subject>Pituitary Gland - drug effects</subject><subject>Pituitary Gland - metabolism</subject><subject>Pyrrolidonecarboxylic Acid - analogs & derivatives</subject><subject>Radioimmunoassay</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Reference Values</subject><subject>Thyroid</subject><subject>Thyroid Gland - drug effects</subject><subject>Thyroid Gland - metabolism</subject><subject>Thyroidectomy</subject><subject>Thyrotropin - blood</subject><subject>Thyrotropin-releasing hormone</subject><subject>Thyrotropin-Releasing Hormone - analogs & derivatives</subject><subject>Thyrotropin-Releasing Hormone - blood</subject><subject>Thyrotropin-Releasing Hormone - metabolism</subject><subject>Thyrotropin-Releasing Hormone - pharmacology</subject><subject>Thyroxine - blood</subject><subject>Thyroxine - pharmacology</subject><subject>Triiodothyronine - blood</subject><subject>Triiodothyronine - pharmacology</subject><subject>Vertebrates: endocrinology</subject><issn>0196-9781</issn><issn>1873-5169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1rFDEUhoNY6lr9Bwq5EFHoaLLJ5ONGKEVboSDIeh0yyRk2kpmMyUxh_73Z7rCXSi5ycZ7zkjwvQm8o-UQJFZ8J1aLRUtEPSn_UhGjakGdoQ5VkTUuFfo42Z-QFelnKb0II51pdosttS6gUaoP8bn_IKXi8T3lII-Ah-SXaOaQRpx7vft7jKYNbckkZR3iEWHAYcbYz3h-mNO9ttMNSrvEU5iXMNh-u8bxG2tHjLqbkX6GL3sYCr9f7Cv369nV3e988_Lj7fnvz0DhO5dx0qnWqJVwq6CR0vRO6d1JzBkwB64TVvWSt2PYtI-C53CrJe9YxYvUWqJTsCr0_5U45_VmgzGYIxUGMdoS0FCM1UYzW8z-QtlUQk7yC_AS6nErJ0Jsph6H-0lBiji2Yo2JzVGyUNk8tGFLX3q75SzeAPy-t2uv83Tq3xdnYZzu6UM6YqIwUumJfTli1Do8BsikuwOjAh9rJbHwK_37HXxn6o7g</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>Simard, Marie</creator><creator>Pekary, A.Eugene</creator><creator>Smith, Vierka P.</creator><creator>Hershman, Jerome M.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>1989</creationdate><title>Thyroid hormone modulation of TRH precursor levels in rat hypothalamus, pituitary, thyroid and blood</title><author>Simard, Marie ; Pekary, A.Eugene ; Smith, Vierka P. ; Hershman, Jerome M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-b85c850478eb7ebfc69fc7943e38e3b6a9f73562f530ed472874f3b30a92e1773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Chromatography</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects. Hormone interactions. Hormone actions on several organ systems. Adaptive reactions</topic><topic>Hypothalamus</topic><topic>Hypothalamus - drug effects</topic><topic>Hypothalamus - metabolism</topic><topic>Hypothyroidism - blood</topic><topic>Male</topic><topic>Pituitary</topic><topic>Pituitary Gland - drug effects</topic><topic>Pituitary Gland - metabolism</topic><topic>Pyrrolidonecarboxylic Acid - analogs & derivatives</topic><topic>Radioimmunoassay</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Reference Values</topic><topic>Thyroid</topic><topic>Thyroid Gland - drug effects</topic><topic>Thyroid Gland - metabolism</topic><topic>Thyroidectomy</topic><topic>Thyrotropin - blood</topic><topic>Thyrotropin-releasing hormone</topic><topic>Thyrotropin-Releasing Hormone - analogs & derivatives</topic><topic>Thyrotropin-Releasing Hormone - blood</topic><topic>Thyrotropin-Releasing Hormone - metabolism</topic><topic>Thyrotropin-Releasing Hormone - pharmacology</topic><topic>Thyroxine - blood</topic><topic>Thyroxine - pharmacology</topic><topic>Triiodothyronine - blood</topic><topic>Triiodothyronine - pharmacology</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simard, Marie</creatorcontrib><creatorcontrib>Pekary, A.Eugene</creatorcontrib><creatorcontrib>Smith, Vierka P.</creatorcontrib><creatorcontrib>Hershman, Jerome M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Peptides (New York, N.Y. : 1980)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simard, Marie</au><au>Pekary, A.Eugene</au><au>Smith, Vierka P.</au><au>Hershman, Jerome M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thyroid hormone modulation of TRH precursor levels in rat hypothalamus, pituitary, thyroid and blood</atitle><jtitle>Peptides (New York, N.Y. : 1980)</jtitle><addtitle>Peptides</addtitle><date>1989</date><risdate>1989</risdate><volume>10</volume><issue>1</issue><spage>145</spage><epage>155</epage><pages>145-155</pages><issn>0196-9781</issn><eissn>1873-5169</eissn><coden>PPTDD5</coden><abstract>In the present study we have examined the in vivo effects of thyroid hormones and TRH on tissue and blood levels of TRH and TRH-Gly (pGlu-His-Pro-Gly), a TRH precursor. Using specific radioimmunoassays (RIAs), we measured TRH immunoreactivity (TRH-IR) and TRH-Gly-IR concentrations in blood, hypothalamus, anterior and posterior pituitary, and thyroid in euthyroid, hypothyroid and thyroxine (T4)-treated 250 g male Sprague-Dawley rats. TRH-Gly-IR and TRH-IR were detected in all of these tissues. Highly significant positive correlations between whole blood TRH-Gly-IR levels and the corresponding serum TSH values (
p<0.01), whole blood TRH-IR versus serum TSH (
p<0.01) and whole blood TRH-Gly-IR versus whole blood TRH-IR (
p<0.01) are consistent with cosecretion of TRH and TRH precursor peptides into the circulation. Euthyroid rats injected with TRH IP (1 μg/100 g b.wt.) and hypothyroid rats had 4-fold higher whole blood TRH-Gly-IR levels compared to euthyroid controls (
p<0.0005). Injection of TRH into euthyroid rats significantly increased the TRH-Gly-IR concentration in the hypothalamus, anterior and posterior pituitary and thyroid. The increase in blood TRH-Gly-IR following intravenous TRH may be due, in part, to partial saturation of TRH-degrading enzymes in blood and cell membranes. The ratio of TRH-Gly to TRH was significantly increased in the anterior pituitary by hypothyroidism and TRH injection, suggesting that thyroid hormones and TRH regulate the alpha-amidation of TRH-Gly to form TRH in this tissue. TRH-Gly levels of pooled pituitary and thyroid extracts quantitated by a combination of TRH-Gly RIA and high performance liquid chromatography (HPLC) revealed several-fold increases following incubation at 60°C. Heating at this temperature may block the alpha-amidation activity in extra-hypothalamic tissues but not the “trypsin-like” enzymes which cleave prepro-TRH into TRH-Gly-immunoreactive peptides.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>2501768</pmid><doi>10.1016/0196-9781(89)90091-0</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0196-9781 |
| ispartof | Peptides (New York, N.Y. : 1980), 1989, Vol.10 (1), p.145-155 |
| issn | 0196-9781 1873-5169 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79083131 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biological and medical sciences Blood Chromatography Fundamental and applied biological sciences. Psychology General aspects. Hormone interactions. Hormone actions on several organ systems. Adaptive reactions Hypothalamus Hypothalamus - drug effects Hypothalamus - metabolism Hypothyroidism - blood Male Pituitary Pituitary Gland - drug effects Pituitary Gland - metabolism Pyrrolidonecarboxylic Acid - analogs & derivatives Radioimmunoassay Rats Rats, Inbred Strains Reference Values Thyroid Thyroid Gland - drug effects Thyroid Gland - metabolism Thyroidectomy Thyrotropin - blood Thyrotropin-releasing hormone Thyrotropin-Releasing Hormone - analogs & derivatives Thyrotropin-Releasing Hormone - blood Thyrotropin-Releasing Hormone - metabolism Thyrotropin-Releasing Hormone - pharmacology Thyroxine - blood Thyroxine - pharmacology Triiodothyronine - blood Triiodothyronine - pharmacology Vertebrates: endocrinology |
| title | Thyroid hormone modulation of TRH precursor levels in rat hypothalamus, pituitary, thyroid and blood |
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