Plasmid localization and mapping of two Azospirillum brasilense loci that affect exopolysaccharide synthesis
Two Azospirillum brasilense loci that correct Rhizobium meliloti exoB and exoC mutants for exopolysaccharide (EPS) synthesis have been identified previously (K. W. Michiels, J. Vanderleyden, A. P. Van Gool, E. R. Signer, J. Bacteriol., 1988b). A. brasilense exo mutants produce EPS of lower molecular...
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Veröffentlicht in: | Plasmid 1989-03, Vol.21 (2), p.142-146 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Two
Azospirillum brasilense loci that correct
Rhizobium meliloti
exoB and
exoC mutants for exopolysaccharide (EPS) synthesis have been identified previously (K. W. Michiels, J. Vanderleyden, A. P. Van Gool, E. R. Signer,
J. Bacteriol., 1988b).
A. brasilense
exo mutants produce EPS of lower molecular weight than the wild type strain. Here, we show by hybridization that these
exo loci are located on a 90-MDa plasmid in
A. brasilense Sp7. In four other
Azospirillum strains but not in
A. lipoferum SpBr17, the loci are likewise located on a plasmid of approximately the same size. Transposon Tn
5 insertions in these loci were isolated and mapped on the cloned DNA by restriction analysis. Hybridization of restriction digests of purified 90-MDa plasmid DNA with probes containing the
exo loci confirmed their plasmid location. This is the first report on plasmid localization of genes in
Azospirillum. |
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ISSN: | 0147-619X 1095-9890 |
DOI: | 10.1016/0147-619X(89)90058-9 |