Large scale preparation of bacteriophage λ by tangential flow ultrafiltration for isolation of λ DNA

A preparative procedure for purifying bacteriophage λ from large volumes of phage lysates by recirculating tangential flow ultrafiltration is described. λ DNA, isolated by deproteinization of the phage, is suitable for use in molecular biology.

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Bibliographische Detailangaben
Veröffentlicht in:	Analytical biochemistry 1989-02, Vol.176 (2), p.373-374
Hauptverfasser:	Rembhotkar, G.W., Khatri, G.S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophage lambda - isolation & purification Biological and medical sciences DNA DNA, Viral - isolation & purification Fundamental and applied biological sciences. Psychology Membranes, Artificial Microbiology Miscellaneous Molecular Weight Ultrafiltration Virology
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	A preparative procedure for purifying bacteriophage λ from large volumes of phage lysates by recirculating tangential flow ultrafiltration is described. λ DNA, isolated by deproteinization of the phage, is suitable for use in molecular biology.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(89)90326-6