Preparation of plant DNA for separation by pulsed field gel electrophoresis

Preparation of plant DNA for separation by pulsed field gel electrophoresis

A method was developed for the preparation of completely intact plant DNA embedded in agarose, and suitable for restriction enzyme digestion. Digestion with restriction enzyme was carried out according to modified protocols of Anand [1] and Kenwrick et al. [2]. The new method of DNA isolation allows...

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Veröffentlicht in:Electrophoresis 1989, Vol.10 (4), p.267-268
Hauptverfasser: Devos, Katrien M., Vercruysse-Dewitte, Daisy
Format: Artikel
Sprache:eng
Schlagworte:
Blotting, Southern
DNA - isolation & purification
Electrophoresis - methods
Molecular Weight
Plants - genetics
Protoplasts - analysis
Specimen Handling
Triticum - genetics
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Zusammenfassung:A method was developed for the preparation of completely intact plant DNA embedded in agarose, and suitable for restriction enzyme digestion. Digestion with restriction enzyme was carried out according to modified protocols of Anand [1] and Kenwrick et al. [2]. The new method of DNA isolation allows the separation of high molecular weight plant DNA by pulsed field gel electrophoresis.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.1150100410