Identification and characterization of the BPV-2 L2 protein

The bovine papilloma virus type 2 (BPV-2) L2 open reading frame was cloned into a λ pL promoter expression vector. This plasmid was shown to express a fusion protein which constituted 75% of the BPV-2 L2 ORF linked to the first 13 N-terminal amino acids of the λ cll gene product. Antisera generated...

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Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 1989-07, Vol.171 (1), p.298-301
Hauptverfasser: Rippe, Richard A., Meinke, William J.
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Sprache:eng
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Zusammenfassung:The bovine papilloma virus type 2 (BPV-2) L2 open reading frame was cloned into a λ pL promoter expression vector. This plasmid was shown to express a fusion protein which constituted 75% of the BPV-2 L2 ORF linked to the first 13 N-terminal amino acids of the λ cll gene product. Antisera generated against this fusion protein were used to identify the L2 gene product as a 64,000-Da protein in BPV-2 virions. Western blot analysis demonstrated that the L2 viral protein was present in full capsids and in small amounts in empty capsids. Densitometer analysis indicated that the L2 protein constituted only 8% of the total l1 + L2 protein content of full capsids. Antisera was also used to demonstrate that the BPV-2 L2 protein is antigenically related to the BPV-1 L2 protein.
ISSN:0042-6822
1096-0341
DOI:10.1016/0042-6822(89)90543-6