Growth hormone (GH) assays: influence of standard preparations, GH isoforms, assay characteristics, and GH-binding protein
The impact of the adoption of the new biosynthetic growth hormone (GH) WHO International Reference Preparation (IRP 88/624), and the recommendation to report results in microgram/L instead of mU/L, is described. Conversion factors were determined by comparing both the linear and nonlinear relations...
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| Veröffentlicht in: | Clinical chemistry (Baltimore, Md.) Md.), 1997-06, Vol.43 (6), p.950-956 |
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| container_title | Clinical chemistry (Baltimore, Md.) |
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| creator | Jansson, Chatarina Boguszewski, Cesar Rosberg, Sten Carlsson, Lena Albertsson-Wikland, Kerstin |
| description | The impact of the adoption of the new biosynthetic growth hormone (GH) WHO International Reference Preparation (IRP 88/624), and the recommendation to report results in microgram/L instead of mU/L, is described. Conversion factors were determined by comparing both the linear and nonlinear relations of the GH values. The Pharmacia polyclonal IRMA (p-IRMA) and the DELFIA monoclonal time-resolved immunofluorometric assay (trIFMA) with kit calibrators calibrated either against the pituitary-derived WHO IRP 80/505 or the new 88/624 were evaluated. Conversion factors of 4.17 mU/L = 1 microgram/L for the p-IRMA and 4.31 mU/L = 1 microgram/L for the trIFMA were necessary. Different cross-reactivity patterns for the deaminated and dimer 22-kDa, 20-kDa, and 17-kDa GH isoforms were found. Expected GH recovery was similar when the measured values were adjusted according to the results of the cross-reactivity study. |
| doi_str_mv | 10.1093/clinchem/43.6.950 |
| format | Article |
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Expected GH recovery was similar when the measured values were adjusted according to the results of the cross-reactivity study.</description><identifier>ISSN: 0009-9147</identifier><identifier>EISSN: 1530-8561</identifier><identifier>DOI: 10.1093/clinchem/43.6.950</identifier><identifier>PMID: 9191545</identifier><language>eng</language><publisher>England: Am Assoc Clin Chem</publisher><subject>Adolescent ; Antibodies ; Antibodies, Monoclonal ; Carrier Proteins - blood ; Child ; Female ; Fluoroimmunoassay ; Human Growth Hormone - blood ; Humans ; Isomerism ; Reagent Kits, Diagnostic ; Reference Standards ; World Health Organization</subject><ispartof>Clinical chemistry (Baltimore, Md.), 1997-06, Vol.43 (6), p.950-956</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-33fdee1b87972f2a182d3dc21e50c61c4f72eca5f1dcf394456d298c00f178e53</citedby><cites>FETCH-LOGICAL-c369t-33fdee1b87972f2a182d3dc21e50c61c4f72eca5f1dcf394456d298c00f178e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9191545$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jansson, Chatarina</creatorcontrib><creatorcontrib>Boguszewski, Cesar</creatorcontrib><creatorcontrib>Rosberg, Sten</creatorcontrib><creatorcontrib>Carlsson, Lena</creatorcontrib><creatorcontrib>Albertsson-Wikland, Kerstin</creatorcontrib><title>Growth hormone (GH) assays: influence of standard preparations, GH isoforms, assay characteristics, and GH-binding protein</title><title>Clinical chemistry (Baltimore, Md.)</title><addtitle>Clin Chem</addtitle><description>The impact of the adoption of the new biosynthetic growth hormone (GH) WHO International Reference Preparation (IRP 88/624), and the recommendation to report results in microgram/L instead of mU/L, is described. Conversion factors were determined by comparing both the linear and nonlinear relations of the GH values. The Pharmacia polyclonal IRMA (p-IRMA) and the DELFIA monoclonal time-resolved immunofluorometric assay (trIFMA) with kit calibrators calibrated either against the pituitary-derived WHO IRP 80/505 or the new 88/624 were evaluated. Conversion factors of 4.17 mU/L = 1 microgram/L for the p-IRMA and 4.31 mU/L = 1 microgram/L for the trIFMA were necessary. Different cross-reactivity patterns for the deaminated and dimer 22-kDa, 20-kDa, and 17-kDa GH isoforms were found. Expected GH recovery was similar when the measured values were adjusted according to the results of the cross-reactivity study.</description><subject>Adolescent</subject><subject>Antibodies</subject><subject>Antibodies, Monoclonal</subject><subject>Carrier Proteins - blood</subject><subject>Child</subject><subject>Female</subject><subject>Fluoroimmunoassay</subject><subject>Human Growth Hormone - blood</subject><subject>Humans</subject><subject>Isomerism</subject><subject>Reagent Kits, Diagnostic</subject><subject>Reference Standards</subject><subject>World Health Organization</subject><issn>0009-9147</issn><issn>1530-8561</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEFrGzEQhUVJcJy0P6CHgC4JLWQdabXaXeVWTGoXArm0ZyFLI6_KruRKaxb310epXZ-GmffeN_AQ-kzJghLBHnXvvO5geKzYol4ITj6gOeWMFC2v6QWaE0JEIWjVXKHrlH7ntWraeoZmggrKKz5Hf1cxTGOHuxCH4AF_Wa2_YpWSOqQn7Lzt9-A14GBxGpU3Khq8i7BTUY0u-PSAV2vsUrA5npd_Qay7LOsRokuj0-9nb7Kv2DhvnN9mQBjB-Y_o0qo-wafTvEG_vj__XK6Ll9fVj-W3l0KzWowFY9YA0E3biKa0paJtaZjRJQVOdE11ZZsStOKWGm2ZqCpem1K0mhBLmxY4u0H3R27--2cPaZSDSxr6XnkI-yQbQRpW8zYb6dGoY0gpgpW76AYVD5IS-d63_N-3rJisZe47Z25P8P1mAHNOnArO-t1R79y2m1wEmQbV99lN5TRNZ84bxSSMSw</recordid><startdate>19970601</startdate><enddate>19970601</enddate><creator>Jansson, Chatarina</creator><creator>Boguszewski, Cesar</creator><creator>Rosberg, Sten</creator><creator>Carlsson, Lena</creator><creator>Albertsson-Wikland, Kerstin</creator><general>Am Assoc Clin Chem</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970601</creationdate><title>Growth hormone (GH) assays: influence of standard preparations, GH isoforms, assay characteristics, and GH-binding protein</title><author>Jansson, Chatarina ; Boguszewski, Cesar ; Rosberg, Sten ; Carlsson, Lena ; Albertsson-Wikland, Kerstin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-33fdee1b87972f2a182d3dc21e50c61c4f72eca5f1dcf394456d298c00f178e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adolescent</topic><topic>Antibodies</topic><topic>Antibodies, Monoclonal</topic><topic>Carrier Proteins - blood</topic><topic>Child</topic><topic>Female</topic><topic>Fluoroimmunoassay</topic><topic>Human Growth Hormone - blood</topic><topic>Humans</topic><topic>Isomerism</topic><topic>Reagent Kits, Diagnostic</topic><topic>Reference Standards</topic><topic>World Health Organization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jansson, Chatarina</creatorcontrib><creatorcontrib>Boguszewski, Cesar</creatorcontrib><creatorcontrib>Rosberg, Sten</creatorcontrib><creatorcontrib>Carlsson, Lena</creatorcontrib><creatorcontrib>Albertsson-Wikland, Kerstin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jansson, Chatarina</au><au>Boguszewski, Cesar</au><au>Rosberg, Sten</au><au>Carlsson, Lena</au><au>Albertsson-Wikland, Kerstin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth hormone (GH) assays: influence of standard preparations, GH isoforms, assay characteristics, and GH-binding protein</atitle><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle><addtitle>Clin Chem</addtitle><date>1997-06-01</date><risdate>1997</risdate><volume>43</volume><issue>6</issue><spage>950</spage><epage>956</epage><pages>950-956</pages><issn>0009-9147</issn><eissn>1530-8561</eissn><abstract>The impact of the adoption of the new biosynthetic growth hormone (GH) WHO International Reference Preparation (IRP 88/624), and the recommendation to report results in microgram/L instead of mU/L, is described. Conversion factors were determined by comparing both the linear and nonlinear relations of the GH values. The Pharmacia polyclonal IRMA (p-IRMA) and the DELFIA monoclonal time-resolved immunofluorometric assay (trIFMA) with kit calibrators calibrated either against the pituitary-derived WHO IRP 80/505 or the new 88/624 were evaluated. Conversion factors of 4.17 mU/L = 1 microgram/L for the p-IRMA and 4.31 mU/L = 1 microgram/L for the trIFMA were necessary. Different cross-reactivity patterns for the deaminated and dimer 22-kDa, 20-kDa, and 17-kDa GH isoforms were found. Expected GH recovery was similar when the measured values were adjusted according to the results of the cross-reactivity study.</abstract><cop>England</cop><pub>Am Assoc Clin Chem</pub><pmid>9191545</pmid><doi>10.1093/clinchem/43.6.950</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical chemistry (Baltimore, Md.), 1997-06, Vol.43 (6), p.950-956 |
| issn | 0009-9147 1530-8561 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Adolescent Antibodies Antibodies, Monoclonal Carrier Proteins - blood Child Female Fluoroimmunoassay Human Growth Hormone - blood Humans Isomerism Reagent Kits, Diagnostic Reference Standards World Health Organization |
| title | Growth hormone (GH) assays: influence of standard preparations, GH isoforms, assay characteristics, and GH-binding protein |
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