The 9-fluorenylmethyloxycarbonyl group as a 5′-OH protection in oligonucleotide synthesis

Oligo‐DNAs are synthesized on a solid support using the 9‐fluorenylmethyloxycarbonyl group as a 5′‐OH base labile protection. The synthesis of the pure protected nucleotides, a relevant phosphoramidite‐type strategy of coupling, and the optimization of the deprotection steps are described. This new synthetic method is an alternative to the standard protocol that avoids acidic conditions.