Rational design of nematode vaccines: Hidden antigens

Hidden antigens are defined and the general validity of the hidden antigen approach is considered. Approaches to the problem of identifying hidden antigens are offered. The nature of the immune responses induced by injection of hidden antigens and their value in giving protection is considered in the light of the site of the hidden antigen in vivo. Particular attention is given to the value of integral membrane ectoenzymes as protective hidden antigens. The need to generate hidden antigens as recombinant proteins and the possibilities and problems associated with linear, conformational and carbohydrate epitopes are outlined. Finally, concerns about the lack of stimulation of induced immune responses and the risks of resistance developing are addressed.