Tolerance of class I histocompatibility antigens expressed extrathymically

ALTHOUGH convincing evidence has been obtained for the imposition of self-tolerance by the intrathymic deletion of self-reactive T cells 1–4 , the development of tolerance to antigens which are expressed only in the periphery is not so well understood. We have approached this question by creating tr...

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Veröffentlicht in:Nature (London) 1989-06, Vol.339 (6226), p.622-624
Hauptverfasser: Morahan, Grant, Allison, Janette, Miller, J. F. A. P
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Sprache:eng
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Zusammenfassung:ALTHOUGH convincing evidence has been obtained for the imposition of self-tolerance by the intrathymic deletion of self-reactive T cells 1–4 , the development of tolerance to antigens which are expressed only in the periphery is not so well understood. We have approached this question by creating transgenic mice 5 which carry a class I major histocompatibility complex (MHC) gene (H-2K b ) linked to the rat insulin promoter 6 . Mice expressing the transgene develop diabetes, but do not appear to mount an immune response against the transgene-expressing pancreaticβ-cells, even when the transgene is allogeneic with respect to the endogenous host H-2 antigens 6 . We have now explored the mechanism of this tolerance further. We find that spleen cells from pre-diabetic transgenic (RIP-K b ) mice do not kill targets bearing H-2K b , whereas thymus cells from the same mice do. The unresponsiveness of these spleen cells can be reversed in vitro by providing recombinant interleukin-2 (rIL-2). In older, diabetic mice, responsiveness develops as the pancreatic β-cells are lost. Our results point to an extrathymic mechanism of tolerance induction, dependent on the continuous presence of antigen and the lack of IL-2 in the local environment of potentially reactive T cells.
ISSN:0028-0836
1476-4687
DOI:10.1038/339622a0