Surface plasmon resonance analysis of topoisomerase I-DNA binding: effect of Mg2+ and DNA sequence

Surface plasmon resonance analysis of topoisomerase I-DNA binding: effect of Mg2+ and DNA sequence

Surface plasmon resonance detection was used to characterize interactions of human topoisomerase I and DNA. The disassociation of topoisomerase I and DNA is characterized by two rate constants. This suggests two parallel but independent pathways of release. DNA association appears to be complex. Mg...

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Veröffentlicht in:Anti-cancer drugs 1997-04, Vol.8 (4), p.336-344
Hauptverfasser: Pond, Christopher D, Holden, Joseph A, Schnabel, Peter C, Barrows, Louis R
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Base Sequence
Biosensing Techniques
Catalysis
DNA - metabolism
DNA Topoisomerases, Type I - metabolism
DNA, Bacterial - metabolism
Kinetics
Magnesium - pharmacology
Male
Salmon
Spectrum Analysis - methods
Substrate Specificity
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Zusammenfassung:Surface plasmon resonance detection was used to characterize interactions of human topoisomerase I and DNA. The disassociation of topoisomerase I and DNA is characterized by two rate constants. This suggests two parallel but independent pathways of release. DNA association appears to be complex. Mg was found to increase both disassociation rate constants and may have a detectable effect on topoisomerase I DNA association. DNA base content did not affect disassociation rate constants or the rate of association.
ISSN:0959-4973
1473-5741
DOI:10.1097/00001813-199704000-00005