Simultaneous determination of the colorants Sunset Yellow FCF and Quinoline Yellow by solid-phase spectrophotometry using partial least squares multivariate calibration

A method for the simultaneous determination of the colorants Sunset Yellow FCF and Quinoline Yellow using solid-phase spectrophotometry is proposed. The colorants were isolated in Sephadex DEAE A-25 gel at pH 5.0, the gel-colorants system was packed in a 1 mm silica cell and spectra were recorded between 400 and 600 nm against a blank. Statistical results were obtained by partial least squares (PLS) multivariate calibration. The optimized matrix by using the PLS-2 method enables the determination of the colorants in artificial mixtures and commercial soft drinks.