Identification of porcine Pneumocystis carinii as a genetically distinct organism by DNA amplification

DNA was amplified from lung samples from three piglets infected with Pneumocystis carinii, using oligonucleotide primers designed to the P. carinii mitochondrial large subunit ribosomal RNA gene. The nucleotide sequence of the amplification product was determined and indicated lack of sequence varia...

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Veröffentlicht in:	APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 1997-04, Vol.105 (4), p.317-321
Hauptverfasser:	Wakefield, A.E, Keely, S.P, Stringer, J.R, Christensen, C.B.V, Ahrens, P, Peters, S.E, Bille-Hansen, V, Henriksen, S.Aa, Jorsal, S.E, Settnes, O.P
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences CERDO Cloning, Molecular DNA amplification DNA, Fungal - analysis DNA, Fungal - chemistry DNA, Ribosomal - analysis DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology Humans Life cycle. Host-agent relationship. Pathogenesis Lung - pathology Lung - virology Mice Molecular Sequence Data NUCLEOTIDE SEQUENCE pig PNEUMOCYSTIS Pneumocystis - classification Pneumocystis - genetics Pneumocystis - isolation & purification Pneumocystis carinii Pneumonia, Pneumocystis - physiopathology Pneumonia, Pneumocystis - veterinary Pneumonia, Pneumocystis - virology Polymerase Chain Reaction - methods PORCIN Protozoa Rats RNA - genetics RNA, Ribosomal - genetics SECUENCIA NUCLEOTIDICA Sequence Homology, Nucleic Acid SEQUENCE NUCLEOTIDIQUE SWINE Swine Diseases
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Zusammenfassung:	DNA was amplified from lung samples from three piglets infected with Pneumocystis carinii, using oligonucleotide primers designed to the P. carinii mitochondrial large subunit ribosomal RNA gene. The nucleotide sequence of the amplification product was determined and indicated lack of sequence variation among these pig‐derived P. carinii samples at this locus. The data showed that porcine P. carinii was genetically distinct from P. carinii isolated from other mammalian host species.
ISSN:	0903-4641 1600-0463
DOI:	10.1111/j.1699-0463.1997.tb00576.x