Triplex DNA : fundamentals, advances, and potential applications for gene therapy

The ability to target specific sequences of DNA through oligonucleotide-based triple-helix formation provides a powerful tool for genetic manipulation. Under experimental conditions, triplex DNA can inhibit DNA transcription and replication, generate site-specific mutations, cleave DNA, and induce h...

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Veröffentlicht in:	Journal of molecular medicine (Berlin, Germany) Germany), 1997-04, Vol.75 (4), p.267-282
Hauptverfasser:	CHAN, P. P, GLAZER, P. M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biotechnology DNA - therapeutic use DNA biosynthesis Fundamental and applied biological sciences. Psychology Gene therapy Genetic Therapy - methods Health. Pharmaceutical industry Humans Industrial applications and implications. Economical aspects Nucleic Acid Conformation Oligodeoxyribonucleotides
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Beschreibung
Zusammenfassung:	The ability to target specific sequences of DNA through oligonucleotide-based triple-helix formation provides a powerful tool for genetic manipulation. Under experimental conditions, triplex DNA can inhibit DNA transcription and replication, generate site-specific mutations, cleave DNA, and induce homologous recombination. This review describes the binding requirements for triplex formation, surveys recent advancements in the chemistry and biology of triple helices and considers several potential applications of triplex DNA for use in genetic therapy.
ISSN:	0946-2716 1432-1440
DOI:	10.1007/s001090050112