Role of calcium in the phenylephrine-induced activation of phosphorylase “A” in isolated liver cells

The ability of phenylephrine to activate phosphorylase in liver cells with variable degrees of Ca 2+ loading was studied. Phenylephrine has been found to be capable of stimulating phosphorylase at saturating Ca 2+ concentrations that precluded any further action of this ion. Furthermore the degree o...

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Veröffentlicht in:	Biochemical and biophysical research communications 1989-04, Vol.160 (2), p.480-485
Hauptverfasser:	Saz, J.M., Gonzalez-Manchon, C., Ayuso, M.S., Parrilla, R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences calcium Calcium - physiology Cell physiology Cytosol - enzymology Cytosol - metabolism Enzyme Activation - drug effects Fundamental and applied biological sciences. Psychology Liver - drug effects Liver - enzymology Liver - metabolism Male Molecular and cellular biology phenylephrine Phenylephrine - pharmacology phosphorylase A Phosphorylase a - metabolism Phosphorylases - metabolism Rats Rats, Inbred Strains Responses to growth factors, tumor promotors, other factors
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Zusammenfassung:	The ability of phenylephrine to activate phosphorylase in liver cells with variable degrees of Ca 2+ loading was studied. Phenylephrine has been found to be capable of stimulating phosphorylase at saturating Ca 2+ concentrations that precluded any further action of this ion. Furthermore the degree of activation was proportional to the cellular calcium content. These results allow to conclude that α-adrenergic agonists activate phosphorylase by a mechanism apparently unrelated to their ability to mobilize and subsequently increase the cytosolic concentration of free Ca 2+.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(89)92458-3