Saccharomyces cerevisiae Cell Lysis Mutations cly5 and cly7 Define Temperature‐Sensitive Alleles of PKC1, the Gene Encoding Yeast Protein Kinase C

A set of temperature‐sensitive Saccharomyces cerevisiae mutants designated cly (for cell lysis) 1–8 because the cells lyse at high temperature was isolated in a large screen for yeast temperature‐sensitive mutations (Hartwell, 1967). Here we report the isolation of two plasmids, containing inserts that complement both the cly5 and cly7 mutations. DNA sequencing revealed that both of these inserts contain the gene encoding yeast protein kinase C (PKC1) (Levin et al., 1990). Sequencing of the mutant alleles revealed that cly5 and cly7 contain distinct mutations separated by 194 base pairs. Consistent with this, the cly5 and cly7 ts alleles do not complement each other, and they are genetically linked to PKC1 and to each other. Like other temperature‐sensitive pkc1 alleles, the temperature‐sensitive phenotype is eliminated by growth in high osmotic strength media (Levin and Bartlett‐Heubusch, 1992). © 1997 John Wiley & Sons, Ltd.