Multiple continuous-flow solid-phase peptide synthesis Synthesis of an HIV antigenic peptide and its omission analogues

Multiple continuous‐flow solid‐phase peptide synthesis was performed on a standard polystyrene‐based resin under low‐pressure conditions using a simple manually operated synthesizer. Stable‐flow resin‐packed columns were prepared in small polypropylene flow reactors, adjustable for volume. The concurrent synthesis of 10 peptides was carried out in flow reactors concatenated together; solvents and reactants were passed through this set of columns using moderate overpressure. One decapeptide, H‐Val‐Tyr‐Tyr‐Arg‐Asp‐Ser‐Arg‐Asn‐Pro‐Leu‐NH2, containing an antigenic determinant of the p31 protein product of the pol gene of the human immunodeficiency virus, and its nine omission analogues were synthesized.