Kinked DNA

Kinked DNA

Kinks are thought to be important in DNA-protein interactions but evidence for their existence in free DNA has previously been indirect. The atomic force microscope (AFM) allows direct visualization of molecular structure in situ, and using our newly developed AFM (magnetic a.c. mode or MacMode) we...

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Veröffentlicht in:Nature (London) 1997-04, Vol.386 (6625), p.563-563
Hauptverfasser: Han, Wenhai, Lindsay, S. M, Dlakic, Mensur, Harrington, Rodney E
Format: Artikel
Sprache:eng
Schlagworte:
DNA - chemistry
DNA - ultrastructure
DNA, Circular
Humanities and Social Sciences
Magnesium - chemistry
Microscopy, Atomic Force
multidisciplinary
Nucleic Acid Conformation
Science
Science (multidisciplinary)
scientific-correspondence
Zinc - chemistry
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Beschreibung
Zusammenfassung:Kinks are thought to be important in DNA-protein interactions but evidence for their existence in free DNA has previously been indirect. The atomic force microscope (AFM) allows direct visualization of molecular structure in situ, and using our newly developed AFM (magnetic a.c. mode or MacMode) we have observed small DNA circles change from smoothly bent to abruptly kinked shapes. This effect was dependent on the presence of specific divalent cations. The DNA was bent smoothly in the presence of Mg super(2+), but consisted of nearly straight segments connected by kinks in the presence of Zn super(2+).
ISSN:0028-0836
1476-4687
DOI:10.1038/386563a0