Analysis of N-acetyl-4-O-acetylneuraminic-acid-containing N-linked carbohydrate chains released by peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase F: application to the structure determination of the carbohydrate chains of equine fibrinogen

The carbohydrate chains of equine fibrinogen were enzymatically released by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F. The oligosaccharides obtained were fractionated by a combination of FPLC and HPLC and analyzed by 500-MHz 1H-NMR spectroscopy. Four monosialo and four disialo dian...

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Veröffentlicht in:	European journal of biochemistry 1989-03, Vol.180 (1), p.101-110
Hauptverfasser:	DAMM, J. B. L, VOSHOL, H, HARD, K, KAMERLING, J. P, VLIEGENTHART, J. F. G
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylation Amidohydrolases Animals Biological and medical sciences Blood coagulation. Blood cells Carbohydrate Conformation Carbohydrates - analysis Chromatography, High Pressure Liquid Chromatography, Ion Exchange Coagulation factors Fibrinogen - analysis Fundamental and applied biological sciences. Psychology Horses Magnetic Resonance Spectroscopy Molecular and cellular biology Molecular Structure Oligosaccharides - analysis Peptide Fragments - analysis Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase Sialic Acids - analysis
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Zusammenfassung:	The carbohydrate chains of equine fibrinogen were enzymatically released by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F. The oligosaccharides obtained were fractionated by a combination of FPLC and HPLC and analyzed by 500-MHz 1H-NMR spectroscopy. Four monosialo and four disialo diantennary N-acetyllactosamine type of carbohydrate chains occur: (formula; see text)
ISSN:	0014-2956 1432-1033
DOI:	10.1111/j.1432-1033.1989.tb14620.x