A mitogenic factor from rat muscle

A mitogenic factor from rat muscle

A growth-promoting polypeptide has been purified about 1950-fold from rat abdominal muscle by gel chromatography, isoelectric-focusing (IEF) and reverse phase high pressure liquid chromatographic (RP-HPLC) techniques. This factor is an acidic protein with an isoelectric point (pI) of 3.4 and a mol....

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Veröffentlicht in:Cancer letters 1989-04, Vol.45 (1), p.27-34
Hauptverfasser: Rao, L.S., Rao, S.G.A., Banerji, A.P.
Format: Artikel
Sprache:eng
Schlagworte:
Analytical, structural and metabolic biochemistry
Animals
Binding, Competitive
Biological and medical sciences
Cell Line
Cell Transformation, Neoplastic
Cells, Cultured
Chromatography, Gel
Chromatography, High Pressure Liquid
DNA Replication - drug effects
ErbB Receptors - drug effects
ErbB Receptors - metabolism
Female
Fundamental and applied biological sciences. Psychology
Isoelectric Focusing
mitogenic
Mitogens - isolation & purification
Mitogens - pharmacology
Molecular Weight
Muscles - analysis
Protein hormones. Growth factors. Cytokines
Proteins
Proteins - isolation & purification
Proteins - pharmacology
rat muscle
Rats
Rats, Inbred Strains
transforming factor
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Zusammenfassung:A growth-promoting polypeptide has been purified about 1950-fold from rat abdominal muscle by gel chromatography, isoelectric-focusing (IEF) and reverse phase high pressure liquid chromatographic (RP-HPLC) techniques. This factor is an acidic protein with an isoelectric point (pI) of 3.4 and a mol. wt of 12 kDa when run on SDS-polyacrylamide gels under reducing conditions. It is acid and heat-stable but is sensitive to reducting agent and protease action. It stimulates [ 3H]thymidine incorporation in NRK-49F and NIH 3T3 cells and induces soft agar colony formation of NIH 3T3 cells. The muscle-derived mitogen appears to differ from other known growth factors (GFs) in its physico-chemical properties.
ISSN:0304-3835
1872-7980
DOI:10.1016/0304-3835(89)90032-3