A Fluoroimmunoassay Based on Immunoliposomes Containing Genetically Engineered Lipid-Tagged Antibody

Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with...

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Veröffentlicht in:	Analytical chemistry (Washington) 1997-04, Vol.69 (7), p.1295-1298
Hauptverfasser:	Kobatake, Eiry, Sasakura, Hiroyuki, Haruyama, Tetsuya, Laukkanen, Marja-Leena, Keinänen, Kari, Aizawa, Masuo
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding, Competitive Biochemistry Biological and medical sciences Diverse techniques Fluoresceins Fluorescent Dyes Fluoroimmunoassay Fundamental and applied biological sciences. Psychology Genetic Engineering Haptens Immunity (Disease) Lipids Liposomes - immunology Molecular and cellular biology Oxazolone - analogs & derivatives
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Beschreibung
Zusammenfassung:	Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive fluoroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimized assay conditions, antigen could be determined in the concentration range from 10-7 to 10-9 M.
ISSN:	0003-2700 1520-6882
DOI:	10.1021/ac961162+