Immunoblot fingerprinting Aspergillus fumigatus

A new technique for typing Aspergillus fumigatus is presented. This is based on immunoblot fingerprinting each isolate against a rabbit hyperimmune antiserum raised against A. fumigatus NCTC 2109. All isolates were typable and reproducibility for the 16 antigenic bands which formed the basis of the...

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Veröffentlicht in:	Journal of immunological methods 1989-03, Vol.118 (2), p.179-186
Hauptverfasser:	Burnie, J.P., Matthews, R.C., Clark, I., Milne, L.J.R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens, Fungal - analysis Aspergilloma Aspergillosis - diagnosis Aspergillus fumigatus Aspergillus fumigatus - analysis Aspergillus fumigatus - classification Aspergillus fumigatus - isolation & purification Biological and medical sciences Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunoblot fingerprinting Immunoblotting - methods Molecular immunology Molecular Weight Rabbits Reproducibility of Results Silver Nitrate Staining and Labeling Techniques
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Beschreibung
Zusammenfassung:	A new technique for typing Aspergillus fumigatus is presented. This is based on immunoblot fingerprinting each isolate against a rabbit hyperimmune antiserum raised against A. fumigatus NCTC 2109. All isolates were typable and reproducibility for the 16 antigenic bands which formed the basis of the system was excellent. Discrimination was better than silver staining and revealed 11 types among the 21 isolates from eight patients with an aspergilloma. Each aspergilloma could be due to either a single or multiple types.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(89)90004-5