Characterization of a Novel Glycoprotein Isolated from the Basement Membrane Matrix of the Engelbreth-Holm-Swarm Tumor
A previously undescribed protein has been isolated and purified from the extracellular matrix of the Engelbreth-Holm-Swarm (EHS) tumor, a murine tumor that synthesizes an extensive matrix composed of basement membrane molecules. Molecular characterization of the molecule determined that it is a glyc...
Gespeichert in:
| Veröffentlicht in: | The Journal of biological chemistry 1989-03, Vol.264 (9), p.5141-5147 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5147 |
|---|---|
| container_issue | 9 |
| container_start_page | 5141 |
| container_title | The Journal of biological chemistry |
| container_volume | 264 |
| creator | Robinson, L K Murrah, V A Moyer, M P Rohrbach, D H |
| description | A previously undescribed protein has been isolated and purified from the extracellular matrix of the Engelbreth-Holm-Swarm (EHS) tumor, a murine tumor that synthesizes an extensive matrix composed of basement membrane molecules. Molecular characterization of the molecule determined that it is a glycoprotein with internal disulfide bonds and an isoelectric point of 6.0. Electrophoretic mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the glycoprotein migrated as a diffuse band with a molecular weight of approximately 72,000–80,000. The amino acid composition was significantly different from known basement membrane components. Polyclonal antibodies that specifically recognize the glycoprotein localized it to the kidney glomerular basement membrane. These antibodies did not cross-react with either known basement membrane components (laminin, type IV collagen, and heparan sulfate proteoglycan), with 70K “culture shock” protein or with components of normal mouse serum (including mouse transferrin, albumin, or α-fetoprotein), when analyzed by “Western” immunoblots. Our data indicate that the glycoprotein is synthesized by the EHS tumor cells and is present at relatively high levels in the EHS tumor matrix. |
| doi_str_mv | 10.1016/S0021-9258(18)83710-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78909017</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021925818837103</els_id><sourcerecordid>15289048</sourcerecordid><originalsourceid>FETCH-LOGICAL-c494t-e8bfc3718cf94191f90ed62e4de7d04f91538e3bb89f2e6434b0b057fb44055e3</originalsourceid><addsrcrecordid>eNqFkV9vFCEUxSdGU9fqR2hCjDH6MAoDzMCT0U1tm7T60Jr4RoC5dDAzQwvs9s-nl-1u9rW8kHB-93LvOVV1RPAXgkn79RLjhtSy4eITEZ8F7Qiu6YtqQbCgNeXk78tqsUdeV29S-ofLYZIcVAdNeWwFX1Tr5aCjthmif9TZhxkFhzT6FdYwopPxwYabGDL4GZ2lMOoMPXIxTCgPgH7oBBPMGV3AZKKeAV3oHP39psVGP56vYTQR8lCfhnGqL-90nNDVagrxbfXK6THBu919WP35eXy1PK3Pf5-cLb-f15ZJlmsQxtmymbBOMiKJkxj6tgHWQ9dj5iThVAA1RkjXQMsoM9hg3jnDGOYc6GH1cdu3bHG7gpTV5JOFcSzThlVSnZBYYtI9CxLeFJSJAvItaGNIKYJTN9FPOj4ogtUmGPUUjNq4rohQT8EoWuqOdh-szAT9vmqXRNE_7HSdrB5d8dP6tMc6SghtZcHeb7HBXw93PoIyPtgBJtW0TEnFCSMF-raFoFi79hBVsh5mC30psFn1wT8z7X-JT7YV</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15289048</pqid></control><display><type>article</type><title>Characterization of a Novel Glycoprotein Isolated from the Basement Membrane Matrix of the Engelbreth-Holm-Swarm Tumor</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Robinson, L K ; Murrah, V A ; Moyer, M P ; Rohrbach, D H</creator><creatorcontrib>Robinson, L K ; Murrah, V A ; Moyer, M P ; Rohrbach, D H</creatorcontrib><description>A previously undescribed protein has been isolated and purified from the extracellular matrix of the Engelbreth-Holm-Swarm (EHS) tumor, a murine tumor that synthesizes an extensive matrix composed of basement membrane molecules. Molecular characterization of the molecule determined that it is a glycoprotein with internal disulfide bonds and an isoelectric point of 6.0. Electrophoretic mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the glycoprotein migrated as a diffuse band with a molecular weight of approximately 72,000–80,000. The amino acid composition was significantly different from known basement membrane components. Polyclonal antibodies that specifically recognize the glycoprotein localized it to the kidney glomerular basement membrane. These antibodies did not cross-react with either known basement membrane components (laminin, type IV collagen, and heparan sulfate proteoglycan), with 70K “culture shock” protein or with components of normal mouse serum (including mouse transferrin, albumin, or α-fetoprotein), when analyzed by “Western” immunoblots. Our data indicate that the glycoprotein is synthesized by the EHS tumor cells and is present at relatively high levels in the EHS tumor matrix.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)83710-3</identifier><identifier>PMID: 2925685</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Amino Acids - analysis ; Analytical, structural and metabolic biochemistry ; Animals ; Basement Membrane - analysis ; basement membranes ; Biological and medical sciences ; Carbohydrate Conformation ; Chromatography, DEAE-Cellulose ; Chromatography, Gel ; Disulfides ; Extracellular Matrix - analysis ; Fundamental and applied biological sciences. Psychology ; Glycoproteins ; Glycoproteins - isolation & purification ; Kidney - analysis ; man ; Mice ; Mice, Inbred C57BL ; Molecular Weight ; Neoplasm Proteins - isolation & purification ; Proteins ; Sarcoma, Experimental - analysis ; Sarcoma, Experimental - pathology</subject><ispartof>The Journal of biological chemistry, 1989-03, Vol.264 (9), p.5141-5147</ispartof><rights>1989 © 1989 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-e8bfc3718cf94191f90ed62e4de7d04f91538e3bb89f2e6434b0b057fb44055e3</citedby><cites>FETCH-LOGICAL-c494t-e8bfc3718cf94191f90ed62e4de7d04f91538e3bb89f2e6434b0b057fb44055e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7311369$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2925685$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Robinson, L K</creatorcontrib><creatorcontrib>Murrah, V A</creatorcontrib><creatorcontrib>Moyer, M P</creatorcontrib><creatorcontrib>Rohrbach, D H</creatorcontrib><title>Characterization of a Novel Glycoprotein Isolated from the Basement Membrane Matrix of the Engelbreth-Holm-Swarm Tumor</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>A previously undescribed protein has been isolated and purified from the extracellular matrix of the Engelbreth-Holm-Swarm (EHS) tumor, a murine tumor that synthesizes an extensive matrix composed of basement membrane molecules. Molecular characterization of the molecule determined that it is a glycoprotein with internal disulfide bonds and an isoelectric point of 6.0. Electrophoretic mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the glycoprotein migrated as a diffuse band with a molecular weight of approximately 72,000–80,000. The amino acid composition was significantly different from known basement membrane components. Polyclonal antibodies that specifically recognize the glycoprotein localized it to the kidney glomerular basement membrane. These antibodies did not cross-react with either known basement membrane components (laminin, type IV collagen, and heparan sulfate proteoglycan), with 70K “culture shock” protein or with components of normal mouse serum (including mouse transferrin, albumin, or α-fetoprotein), when analyzed by “Western” immunoblots. Our data indicate that the glycoprotein is synthesized by the EHS tumor cells and is present at relatively high levels in the EHS tumor matrix.</description><subject>Amino Acids - analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Basement Membrane - analysis</subject><subject>basement membranes</subject><subject>Biological and medical sciences</subject><subject>Carbohydrate Conformation</subject><subject>Chromatography, DEAE-Cellulose</subject><subject>Chromatography, Gel</subject><subject>Disulfides</subject><subject>Extracellular Matrix - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins</subject><subject>Glycoproteins - isolation & purification</subject><subject>Kidney - analysis</subject><subject>man</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular Weight</subject><subject>Neoplasm Proteins - isolation & purification</subject><subject>Proteins</subject><subject>Sarcoma, Experimental - analysis</subject><subject>Sarcoma, Experimental - pathology</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9vFCEUxSdGU9fqR2hCjDH6MAoDzMCT0U1tm7T60Jr4RoC5dDAzQwvs9s-nl-1u9rW8kHB-93LvOVV1RPAXgkn79RLjhtSy4eITEZ8F7Qiu6YtqQbCgNeXk78tqsUdeV29S-ofLYZIcVAdNeWwFX1Tr5aCjthmif9TZhxkFhzT6FdYwopPxwYabGDL4GZ2lMOoMPXIxTCgPgH7oBBPMGV3AZKKeAV3oHP39psVGP56vYTQR8lCfhnGqL-90nNDVagrxbfXK6THBu919WP35eXy1PK3Pf5-cLb-f15ZJlmsQxtmymbBOMiKJkxj6tgHWQ9dj5iThVAA1RkjXQMsoM9hg3jnDGOYc6GH1cdu3bHG7gpTV5JOFcSzThlVSnZBYYtI9CxLeFJSJAvItaGNIKYJTN9FPOj4ogtUmGPUUjNq4rohQT8EoWuqOdh-szAT9vmqXRNE_7HSdrB5d8dP6tMc6SghtZcHeb7HBXw93PoIyPtgBJtW0TEnFCSMF-raFoFi79hBVsh5mC30psFn1wT8z7X-JT7YV</recordid><startdate>19890325</startdate><enddate>19890325</enddate><creator>Robinson, L K</creator><creator>Murrah, V A</creator><creator>Moyer, M P</creator><creator>Rohrbach, D H</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19890325</creationdate><title>Characterization of a Novel Glycoprotein Isolated from the Basement Membrane Matrix of the Engelbreth-Holm-Swarm Tumor</title><author>Robinson, L K ; Murrah, V A ; Moyer, M P ; Rohrbach, D H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-e8bfc3718cf94191f90ed62e4de7d04f91538e3bb89f2e6434b0b057fb44055e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Amino Acids - analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Basement Membrane - analysis</topic><topic>basement membranes</topic><topic>Biological and medical sciences</topic><topic>Carbohydrate Conformation</topic><topic>Chromatography, DEAE-Cellulose</topic><topic>Chromatography, Gel</topic><topic>Disulfides</topic><topic>Extracellular Matrix - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoproteins</topic><topic>Glycoproteins - isolation & purification</topic><topic>Kidney - analysis</topic><topic>man</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular Weight</topic><topic>Neoplasm Proteins - isolation & purification</topic><topic>Proteins</topic><topic>Sarcoma, Experimental - analysis</topic><topic>Sarcoma, Experimental - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Robinson, L K</creatorcontrib><creatorcontrib>Murrah, V A</creatorcontrib><creatorcontrib>Moyer, M P</creatorcontrib><creatorcontrib>Rohrbach, D H</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Robinson, L K</au><au>Murrah, V A</au><au>Moyer, M P</au><au>Rohrbach, D H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a Novel Glycoprotein Isolated from the Basement Membrane Matrix of the Engelbreth-Holm-Swarm Tumor</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1989-03-25</date><risdate>1989</risdate><volume>264</volume><issue>9</issue><spage>5141</spage><epage>5147</epage><pages>5141-5147</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>A previously undescribed protein has been isolated and purified from the extracellular matrix of the Engelbreth-Holm-Swarm (EHS) tumor, a murine tumor that synthesizes an extensive matrix composed of basement membrane molecules. Molecular characterization of the molecule determined that it is a glycoprotein with internal disulfide bonds and an isoelectric point of 6.0. Electrophoretic mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the glycoprotein migrated as a diffuse band with a molecular weight of approximately 72,000–80,000. The amino acid composition was significantly different from known basement membrane components. Polyclonal antibodies that specifically recognize the glycoprotein localized it to the kidney glomerular basement membrane. These antibodies did not cross-react with either known basement membrane components (laminin, type IV collagen, and heparan sulfate proteoglycan), with 70K “culture shock” protein or with components of normal mouse serum (including mouse transferrin, albumin, or α-fetoprotein), when analyzed by “Western” immunoblots. Our data indicate that the glycoprotein is synthesized by the EHS tumor cells and is present at relatively high levels in the EHS tumor matrix.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>2925685</pmid><doi>10.1016/S0021-9258(18)83710-3</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1989-03, Vol.264 (9), p.5141-5147 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78909017 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acids - analysis Analytical, structural and metabolic biochemistry Animals Basement Membrane - analysis basement membranes Biological and medical sciences Carbohydrate Conformation Chromatography, DEAE-Cellulose Chromatography, Gel Disulfides Extracellular Matrix - analysis Fundamental and applied biological sciences. Psychology Glycoproteins Glycoproteins - isolation & purification Kidney - analysis man Mice Mice, Inbred C57BL Molecular Weight Neoplasm Proteins - isolation & purification Proteins Sarcoma, Experimental - analysis Sarcoma, Experimental - pathology |
| title | Characterization of a Novel Glycoprotein Isolated from the Basement Membrane Matrix of the Engelbreth-Holm-Swarm Tumor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T01%3A06%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20a%20Novel%20Glycoprotein%20Isolated%20from%20the%20Basement%20Membrane%20Matrix%20of%20the%20Engelbreth-Holm-Swarm%20Tumor&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Robinson,%20L%20K&rft.date=1989-03-25&rft.volume=264&rft.issue=9&rft.spage=5141&rft.epage=5147&rft.pages=5141-5147&rft.issn=0021-9258&rft.eissn=1083-351X&rft.coden=JBCHA3&rft_id=info:doi/10.1016/S0021-9258(18)83710-3&rft_dat=%3Cproquest_cross%3E15289048%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15289048&rft_id=info:pmid/2925685&rft_els_id=S0021925818837103&rfr_iscdi=true |