The Human HIV-1 Rev Binding-Protein hRIP/Rab (HRB) Maps to Chromosome 2q36

The properties described for the human Rev interacting protein (hRIP) (6) and Rev/Rex activation domain-binding protein (Rab) suggest that it may be a potential cellular cofactor for the HIV-1-Rev protein (3). The Rev transactivator is required for nuclear export of unspliced and partly spliced viral mRNA (4). If Rev is not present, these viral mRNAs remain within the nucleus. Rev contains an RNA binding Here we report mapping of the hRIP/Rab gene using fluorescence in situ hybridization (FISH). This method was used for the regional assignment of the hRIP/Rab gene on human metaphase chromosomes. As probe we used a cDNA encoding the entire open reading frame of the Rab gene (2342 bp), present in the vector pBC12/CMV/Rab.