A time-resolved fluorescence anisotropy study of bilayer membranes containing α-tocopherol

A time-resolved fluorescence anisotropy study of bilayer membranes containing α-tocopherol

Rotational mobility in fluid phase dipalmitoylphosphatidylcholine unilamellar vesicles containing α-tocopherol has been studied by time-resolved anisotropy measurements of fluorescence from a diphenylhexatriene-phosphatidylcholine conjugate. The results are analysed using a simple wobbling-in-cone m...

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Veröffentlicht in:Biochemical and biophysical research communications 1989-01, Vol.158 (2), p.386-391
Hauptverfasser: Bisby, Roger H., Birch, David J.S.
Format: Artikel
Sprache:eng
Schlagworte:
alpha -tocopherol
Artificial membranes and reconstituted systems
Biological and medical sciences
CELL MEMBRANES
Fluorescence Polarization
Fundamental and applied biological sciences. Psychology
LECITHINE
LECITHINS
LECITINAS
Lipid Bilayers
MEMBRANAS CELULARES
MEMBRANE CELLULAIRE
Membrane Fluidity
Membrane physicochemistry
Molecular biophysics
OPTICAL PROPERTIES
PHOSPHATIDYL CHOLINES
PROPIEDADES OPTICAS
PROPRIETE OPTIQUE
TOCOFEROLES
TOCOPHEROL
TOCOPHEROLS
Vitamin E
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Zusammenfassung:Rotational mobility in fluid phase dipalmitoylphosphatidylcholine unilamellar vesicles containing α-tocopherol has been studied by time-resolved anisotropy measurements of fluorescence from a diphenylhexatriene-phosphatidylcholine conjugate. The results are analysed using a simple wobbling-in-cone model. The diphenylhexatriene probe shows an increasing order parameter and more restricted wobbling with increasing α-tocopherol content of the membrane. The diffusional rate for wobbling was found not to change significantly.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(89)80059-2