A competitive enzyme-linked immunosorbent assay for detecting Escherichia coli heat-stable enterotoxin and its application to clinical isolates

Enterotoxigenic Escherichia coli (ETEC) are well recognised enteric pathogens. The epidemiology of heat-stable enterotoxin (STa) producing strains has not been established due mainly to difficulties encountered in performing bioassays on a large scale in baby mice. This study describes a competitive...

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Veröffentlicht in:	The Journal of infection 1989, Vol.18 (1), p.59-66
1. Verfasser:	Cryan, Bartley
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Toxins - analysis Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Child, Preschool Diarrhea - microbiology Enterotoxins - analysis Enzyme-Linked Immunosorbent Assay Escherichia coli Escherichia coli - immunology Escherichia coli - isolation & purification Escherichia coli Infections - microbiology Escherichia coli Proteins Fundamental and applied biological sciences. Psychology Humans Infant Infant, Newborn Mice Mice, Inbred BALB C Microbiology Predictive Value of Tests
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Zusammenfassung:	Enterotoxigenic Escherichia coli (ETEC) are well recognised enteric pathogens. The epidemiology of heat-stable enterotoxin (STa) producing strains has not been established due mainly to difficulties encountered in performing bioassays on a large scale in baby mice. This study describes a competitive enzyme-linked immunosorbent assay (ELISA) for detecting STa and its application to clinical isolates. Compared to the bioassay, the ELISA had a specificity of 93·7% and a sensitivity of 90·9%. It detected as little as 1 μg/1 STa. Of 720 E. coli isolates from children with diarrhoea, 69 (9·6%) were positive for STa by ELISA.
ISSN:	0163-4453 1532-2742
DOI:	10.1016/S0163-4453(89)93692-X