Technical improvements of a commercial ELISA to detect antibodies against bovine herpesvirus 1

Prevention and control of the spread of IBR/IPV virus in cattle presumes exact diagnosis even with large sample collections. The requirements are fulfilled firstly by the application of the most sensitive testing methods for virus specific antibodies, such as the enzyme-linked immunosorbent assay (E...

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Veröffentlicht in:	Medical microbiology and immunology 1989-01, Vol.178 (1), p.9-20
Hauptverfasser:	WITTE, K. H, HANNEMANN, P, DOPATKA, H.-D, GIESENDORF, B
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral - analysis Biological and medical sciences Cattle Enzyme-Linked Immunosorbent Assay - veterinary Fundamental and applied biological sciences. Psychology Herpesvirus 1, Bovine - immunology Microbiology Milk - immunology Techniques used in virology Virology
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Beschreibung
Zusammenfassung:	Prevention and control of the spread of IBR/IPV virus in cattle presumes exact diagnosis even with large sample collections. The requirements are fulfilled firstly by the application of the most sensitive testing methods for virus specific antibodies, such as the enzyme-linked immunosorbent assay (ELISA), and secondly by the introduction of bovine milk as a specimen for sampling. Data are presented demonstrating the application of a commercial ELISA system for evaluation of large sample collections and its optimization for this purpose.
ISSN:	0300-8584 1432-1831
DOI:	10.1007/BF00202287