Biochemical characterization of FMDV A10 and A22 subtypes by PAGE and IEF

Both polyacrylamide gel electrophoresis (PAGE) and iso-electric focusing (IEF) have been standardized using the sucrose density gradient purified 146S particles of FMD virus subtypes A10 and A22. Differences in the molecular weights of structural proteins (VP1, VP2 and VP3 of two subtypes (A10 and A...

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Veröffentlicht in:	Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 1997-01, Vol.20 (1), p.95-99
Hauptverfasser:	Nandi, S, Suryanarayana, V V, Banumathi, N, Sen, A K
Format:	Artikel
Sprache:	eng
Schlagworte:	Aphthovirus - chemistry Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Isoelectric Focusing Molecular Weight Viral Structural Proteins - analysis Viral Structural Proteins - isolation & purification
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Beschreibung
Zusammenfassung:	Both polyacrylamide gel electrophoresis (PAGE) and iso-electric focusing (IEF) have been standardized using the sucrose density gradient purified 146S particles of FMD virus subtypes A10 and A22. Differences in the molecular weights of structural proteins (VP1, VP2 and VP3 of two subtypes (A10 and A22) of FMDV have been revealed in PAGE but no appreciable differences in the pI of VP1, VP2 and VP3 is found in IEF.
ISSN:	0147-9571
DOI:	10.1016/s0147-9571(96)00010-0