Multichannel Microchip Electrospray Mass Spectrometry

Microfabricated multiple-channel glass chips were successfully interfaced to an electrospray ionization mass spectrometer (ESI-MS). The microchip device was fabricated by standard photolithographic, wet chemical etching, and thermal bonding procedures. A high voltage was applied individually from each buffer reservoir for spraying sample sequentially from each channel. With the sampling orifice of the MS grounded, it was found that a liquid flow of 100−200 nL/min was necessary to maintain a stable electrospray. The detection limit of the microchip MS experiment for myoglobin was found to be lower than 6 × 10-8 M. Samples in 75% methanol were successfully analyzed with good sensitivity, as were aqueous samples. The parallel multiple-channel microchip system allowed ESI-MS analysis of different samples of standard peptides and proteins in one chip.