Measuring Serotonin Distribution in Live Cells with Three-Photon Excitation

Measuring Serotonin Distribution in Live Cells with Three-Photon Excitation

Tryptophan and serotonin were imaged with infrared illumination by three-photon excitation (3PE) of their native ultraviolet (UV) fluorescence. This technique, established by 3PE cross section measurements of tryptophan and the monoamines serotonin and dopamine, circumvents the limitations imposed b...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 1997-01, Vol.275 (5299), p.530-532
Hauptverfasser: Maiti, S., Shear, Jason B., Williams, R. M., Zipfel, W. R., Webb, Watt W.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological and medical sciences
Cell Survival
Cells
Cytoplasmic Granules - chemistry
Diverse techniques
Dopamine - analysis
Fluorescence
Fundamental and applied biological sciences. Psychology
Imaging
Laser beams
Laser power
Lasers
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Molecular and cellular biology
Molecules
Neurology
Optical filters
Photochemistry
Photons
Rats
Scientific imaging
Serotonin - analysis
Three dimensional imaging
Tryptophan - analysis
Tumor Cells, Cultured
Ultraviolet radiation
Ultraviolet Rays
Wave excitation
Wavelengths
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Beschreibung
Zusammenfassung:Tryptophan and serotonin were imaged with infrared illumination by three-photon excitation (3PE) of their native ultraviolet (UV) fluorescence. This technique, established by 3PE cross section measurements of tryptophan and the monoamines serotonin and dopamine, circumvents the limitations imposed by photodamage, scattering, and indiscriminate background encountered in other UV microscopies. Three-dimensionally resolved images are presented along with measurements of the serotonin concentration (∼50 mM) and content (up to ∼5 × 10$^8$ molecules) of individual secretory granules.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.275.5299.530